Cas9 Antibodies for Immunoprecipitation

2 matching Cas9 antibodies for Immunoprecipitation after applying filters

Click each Cas9 antibody that interests you to view the full Cas9 antibody datasheet.
View only Cas9 Monoclonal Antibodies or Cas9 Polyclonal Antibodies

Immunoprecipitation - Use Filter to switch application

Supplier data
Origene TA811327 Cas9 antibody, TA811327, Origene.

Mouse Mono [clone OTI1C8], 100 ul
Hu
IP

Supplier data
Novus Biologicals NBP2-52398

Cas9 antibody, NBP2-52398, Novus Biologicals. Immunoprecipitation: CRISPR-Cas9 Antibody (6G12) - C-terminus [NBP2-52398] - CRISPR-Cas9 Antibody (6G12) - C-Terminus [NBP2-52398] - HEK293 cells expressing Flag-SpCas9 were lysed under native conditions. SpCas9 was immunoprecipitated at 4C from 300 ug of whole cell lysate with the 6G12 antibody and a 1:1 mixture of protein A/G sepharose. After 4x washing, the bound proteins were boiled off the beads, separated by 7.5% SDS-PAGE and transfered to nitrocellulose membranes, and SpCas9 was detected

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

Cas9 antibody, NBP2-52398, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP,

Cas9 antibody, NBP2-52398, Novus Biologicals, Immunocytochemistry image

ICC,

Cas9 antibody, NBP2-52398, Novus Biologicals, Immunofluorescence image

IF,

Cas9 antibody, NBP2-52398, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-52717

Cas9 antibody, NBP2-52717, Novus Biologicals. Immunoprecipitation: CRISPR-Cas9 Antibody [NBP2-52717] - HEK293T were transfected with either WT CAS9 plasmid; CAS9 nickase plasmid (GE100019); or negative control vector (PS100001). Input: 10ug total cell lysates; WB was performed (1:2000). IP: 100ug of cell lysates were precipitated with 20ug of NBP2-52717 or control antibody; and 5ug Protein G Beads. Mouse anti-DDK monoclonal antibody was used in WB to detect the immuno-precipitated Cas9 (as Cas9 is Myc-DDK tagged)

Rabbit Poly, 100 ul
Bac
IP, WB

Cas9 antibody, NBP2-52717, Novus Biologicals, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23519

Cas9 antibody, MA5-23519, Invitrogen Antibodies. IP was performed on whole cell extracts (100 µg) from HEK293 cells transfected with a Flag-tagged Cas9 using a CRISPR/Cas9 monoclonal antibody (Product # MA5-23519). The immunoprecipitated proteins were subsequently analyzed by Western Blot with the antibody. Lane 3 and 4 show the result of the IP; a negative IP control (IP on untransfected cells) and the input (15 µg) are shown in lane 2 and 1, respectively.

Mouse Mono [7A9], 50 ug
Hu
ICC, IF, IP, WB

ICC,

Cas9 antibody, MA5-23519, Invitrogen Antibodies, Immunofluorescence image

IF,

Cas9 antibody, MA5-23519, Invitrogen Antibodies, Western Blot image

Supplier data
Cell Signaling Technology 48989S Cas9 antibody, 48989S, Cell Signaling Technology.

Mouse Mono [48989S], 100 ul
Ch
ICC, IF, IP, WB

Cas9 antibody, 48989S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 48989S, Cell Signaling Technology, Immunofluorescence image

IF,

Western Blot

Supplier data
Immuquest IQ651 Cas9 antibody, IQ651, Immuquest. Legend: Western blot - Anti CRISPR/Cas9 Antibody [7A9-3A3]

Mouse Mono [7A9-3A3], 100 ul
Bac
DB, IF, IP, WB

Cas9 antibody, IQ651, Immuquest, Dot Blot image

DB, IF, IP

Supplier data
Novus Biologicals NBP2-52398

Cas9 antibody, NBP2-52398, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody (6G12) - C-terminus [NBP2-52398] - CRISPR-Cas9 Antibody (6G12) - C-Terminus [NBP2-52398] - Control HeLa cells (un-transfected) and HeLa cells expressing Flag-tagged S. pyogenes's CRISPR-Cas9 under the control of PTight (Tet-ON) promoter. Samples were treated for 24 hours with 1ug/uL of Doxycyclin and lysed under native conditions. 30 ug of the whole cell lysate from each sample type per lane was separated by 7.5% SDS-PAGE. Nitrocellulose membrane was incubated

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

ChIP,

ICC,

IF,

Cas9 antibody, NBP2-52398, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-52717 Cas9 antibody, NBP2-52717, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody [NBP2-52717] - HEK293T were transfected with either WT CAS9 plasmid, CAS9 nickase plasmid or control vector. Equivalent amount of each cell lysates (10ug per lane) were immunoblotted with (1:2000).

Cas9 antibody, NBP2-52717, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody [NBP2-52717] - HEK293T were transfected with either WT CAS9 plasmid, CAS9 nickase plasmid or control vector. Equivalent amount of each cell lysates (10ug per lane) were immunoblotted with (1:2000).

Rabbit Poly, 100 ul
Bac
IP, WB

Cas9 antibody, NBP2-52717, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-81124

Cas9 antibody, NBP2-81124, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody (6H4) [NBP2-81124] - Total protein from mock and Cas9 transfected 293 cells, HeLa and 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Cas9 in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Theoretical molecular weight: 158.4 kDa.

Mouse Mono [6H4], 100 ug
Mo
ICC, IF, IP, WB

ICC, IF, IP

Supplier data
Invitrogen Antibodies MA5-23519 Cas9 antibody, MA5-23519, Invitrogen Antibodies. Western Blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9. Figure 2 shows the result of the WB on protein extracts from transfected (lane 2 and 4) and untransfected (lane 1 and 3) cells using a CRISPR/Cas9 monoclonal antibody (Product # MA5-23519) (lane 1 and 2) or an anti-myc antibody (lane 3 and 4).

Cas9 antibody, MA5-23519, Invitrogen Antibodies. Western Blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9. Figure 2 shows the result of the WB on protein extracts from transfected (lane 2 and 4) and untransfected (lane 1 and 3) cells using a CRISPR/Cas9 monoclonal antibody (Product # MA5-23519) (lane 1 and 2) or an anti-myc antibody (lane 3 and 4).

Mouse Mono [7A9], 50 ug
Hu
ICC, IF, IP, WB

ICC,

IF,

Cas9 antibody, MA5-23519, Invitrogen Antibodies, Immunoprecipitation image

Supplier data
Cell Signaling Technology 48989S Cas9 antibody, 48989S, Cell Signaling Technology.

Mouse Mono [48989S], 100 ul
Ch
ICC, IF, IP, WB

ICC,

IF,

Cas9 antibody, 48989S, Cell Signaling Technology, Immunoprecipitation image

Immunofluorescence

Supplier data
Novus Biologicals NBP2-52398

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

ChIP,

ICC,

IP,

Supplier data
Invitrogen Antibodies MA5-23519

Cas9 antibody, MA5-23519, Invitrogen Antibodies. Hela cells were transiently transfected with a Flag-tagged Cas9 expression vector. 48 hours post transfection the cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the Cas9 antibody (Product # MA5-23519) at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT (left). Nuclei were counter-stained with DAPI (right).

Mouse Mono [7A9], 50 ug
Hu
ICC, IF, IP, WB

ICC,

IP,

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

2 matching Cas9 antibodies for Immunoprecipitation from 10 suppliers

Superstar Kits

Cas9 information

Aliases

1 Applications, 5 Species currently selected

2 matching Cas9 antibodies for Immunoprecipitation after applying filters

Immunoprecipitation - Use Filter to switch application

Western Blot

Immunofluorescence

All None
	WB	IF	ICC	FACS	IHC-P	IP	IHC-F	AP	ELISA	ChIP	DB
Bacteria	61	45	43	2	5	4	4		2	2	1
Human	11	4	2	1	1	3	1
Chicken	11	10	9	11	5	1		3
Mouse	2	2	2			2				1
Yeast	1