Antibodies are essential tools in life sciences and are applied in many research areas. However, specificity of mono- and polyclonal antibodies and hence their ability to generate reliable and robust data is under scrutiny in the scientific community. Unspecific binding to non-target antigens may lead to inconclusive or misleading data, and thus to a waste of time, money and samples. Highly specific antibodies are a prerequisite to generate reliable results, emphasizing the urgent need for appropriate validation methods in order to determine antibody specificity and cross-reactivity.
PEPperCHIP® Peptide Microarrays & Services
Antibody Validation
PEPperPRINT offers a number of innovative solutions for antibody validation and in-depth analysis of epitopes for a wide range of antibody formats and isotypes.
These solutions cover a range of applications for e.g.
- Identify linear & conformational epitopes with highest amino acid resolution
- Determine conserved & variable amino acids by full peptide substitution scans
- Analyse cross-reactions and off-targets effects of research, diagnostic and therapeutic antibodies
- Generate antibody fingerprint profiles for seum biomarker discovery
- Human Epitome Microarray with 29, 128 linear human B-cell epitopes
- Epitome-wide antibody profiling
- Analaysis of mono- & polyclonal antibodies, serum & plasma
- Bioinformatic analysis to identify common binding motifs
- Microarray with antigen(s) as overlapping peptides
- Epitope identification with amino acid resolution
- 15 amino acid liner or 7, 10 & 13 amino acid cyclic constrained peptides with max. peptide-peptide overlap
- From single to multiple antigens in a single assay
- Exchange of all amino acid positions of a wild type peptide with the 20 natural amino acids
- Identification of essential conserved & variable amino acid positions
- Unambiquous identification of epitope lengths & epiptope variants
- Assessments of antibody selectivity & cross-reactivity
Step 1: Project initiation
- Describe your scientific aim
- Submit your protein sequence(s) of interest
- Provide your antibody or serum samples
Step 2: Microarray production
- Microarray design
- Peptide printing
- Final quality control
Step 3: PEPperMAP® Service
- On-chip immunoassays
- Microarray read-out
- Data evaluation
Step 4: Scientific Reporting
- Incl. material & methods
- All experimental details, raw and formatted data
- Data discussion & conclusion
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