Cas9 Antibodies for Immunofluorescence

43 matching Cas9 antibodies for Immunofluorescence after applying filters

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View only Cas9 Monoclonal Antibodies or Cas9 Polyclonal Antibodies

Immunofluorescence - Use Filter to switch application

Supplier data
Novus Biologicals NBP2-52398

Cas9 antibody, NBP2-52398, Novus Biologicals. Immunocytochemistry/Immunofluorescence: CRISPR-Cas9 Antibody (6G12) - C-Terminus [NBP2-52398] - CRISPR-Cas9 Antibody (6G12) [NBP2-52398] - Hela cells or Hela cells expressing Flag-tagged SpCas9 under the control of the PTight (Tet-ON) promoter were treated for 24h with 1ug/ul Doxycyclin, fixed and permeabilized with Methanol/Acetone and blocked in 2% BSA in PBS for 2 hours at RT. Cells were stained with 6G12 hybridoma supernatant (diluted 1:10) at 4C o/n, followed by incubation with anti mouse-A

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

Cas9 antibody, NBP2-52398, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP,

Cas9 antibody, NBP2-52398, Novus Biologicals, Immunocytochemistry image

ICC,

Cas9 antibody, NBP2-52398, Novus Biologicals, Immunoprecipitation image

IP,

Cas9 antibody, NBP2-52398, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-52718 Cas9 antibody, NBP2-52718, Novus Biologicals. Immunofluorescence: CRISPR-Cas9 Antibody [NBP2-52718] - HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1ug/mL. Secondary: Donkey anti-Rabbit at 1ug/mL, staining seen in red. Bottom images show GFP stain overlay

Cas9 antibody, NBP2-52718, Novus Biologicals. Immunofluorescence: CRISPR-Cas9 Antibody [NBP2-52718] - HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1ug/mL. Secondary: Donkey anti-Rabbit at 1ug/mL, staining seen in red. Bottom images show GFP stain overlay

Rabbit Poly, 100 ug
Bac
ELISA, IF, WB

ELISA,

Cas9 antibody, NBP2-52718, Novus Biologicals, Western Blot image

Supplier data
Invitrogen Antibodies MA1-202

Cas9 antibody, MA1-202, Invitrogen Antibodies. Immunofluorescent analysis of Cas9 (green) in U2OS cells (left panel) and stable Cas9 expressing U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a CRISPR-Cas9 monoclonal antibody (Product # MA1-202) at a dilution of 5

Mouse Mono [10C11-A12], 100 ug
Hu
FACS, IF, WB

Cas9 antibody, MA1-202, Invitrogen Antibodies, Flow Cytometry image

FACS,

Cas9 antibody, MA1-202, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23519

Cas9 antibody, MA5-23519, Invitrogen Antibodies. Hela cells were transiently transfected with a Flag-tagged Cas9 expression vector. 48 hours post transfection the cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the Cas9 antibody (Product # MA5-23519) at 4°C o/n, followed by incubation with an anti mouse secondary antibody coupled to AF488 for 1 h at RT (left). Nuclei were counter-stained with DAPI (right).

Mouse Mono [7A9], 50 ug
Hu
ICC, IF, IP, WB

ICC,

Cas9 antibody, MA5-23519, Invitrogen Antibodies, Immunoprecipitation image

IP,

Cas9 antibody, MA5-23519, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA1-201

Cas9 antibody, MA1-201, Invitrogen Antibodies. Immunofluorescent analysis of Cas9 (green) in U2OS cells (left panel) and stable Cas9 expressing U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a CRISPR-Cas9 monoclonal antibody (Product # MA1-201) at a dilution of 5

Mouse Mono [7A9-3A3], 100 ug
Hu
IF, WB

Cas9 antibody, MA1-201, Invitrogen Antibodies, Western Blot image

Supplier data
Cell Signaling Technology 19526T Cas9 antibody, 19526T, Cell Signaling Technology.

Rabbit Mono [19526T], 20 ul
Ch
AP, FACS, ICC, IF, IHC-P, WB

AP,

Cas9 antibody, 19526T, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 19526T, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 19526T, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

Cas9 antibody, 19526T, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 14697S Cas9 antibody, 14697S, Cell Signaling Technology.

Mouse Mono [14697S], 100 ul
Ch
FACS, ICC, IF, IHC-P, WB

Cas9 antibody, 14697S, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 14697S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 14697S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

Cas9 antibody, 14697S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 14697T Cas9 antibody, 14697T, Cell Signaling Technology.

Mouse Mono [14697T], 20 ul
Ch
AP, FACS, ICC, IF, IHC-P, WB

AP,

Cas9 antibody, 14697T, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 14697T, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 14697T, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

Cas9 antibody, 14697T, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 19526S Cas9 antibody, 19526S, Cell Signaling Technology.

Rabbit Mono [19526S], 100 ul
Ch
FACS, ICC, IF, IHC-P, WB

Cas9 antibody, 19526S, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 19526S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 19526S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

Cas9 antibody, 19526S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 65832S Cas9 antibody, 65832S, Cell Signaling Technology.

Rabbit Mono [65832S], 100 ul
Ch
FACS, ICC, IF, WB

Cas9 antibody, 65832S, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 65832S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 65832S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 48989S Cas9 antibody, 48989S, Cell Signaling Technology.

Mouse Mono [48989S], 100 ul
Ch
ICC, IF, IP, WB

Cas9 antibody, 48989S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 48989S, Cell Signaling Technology, Immunoprecipitation image

IP,

Cas9 antibody, 48989S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 51610S Cas9 antibody, 51610S, Cell Signaling Technology.

Rabbit Mono [51610S], 100 ul
Ch
AP, FACS, ICC, IF, WB

AP,

Cas9 antibody, 51610S, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 51610S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Cas9 antibody, 51610S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 48796S Cas9 antibody, 48796S, Cell Signaling Technology.

Mouse Mono [48796S], 100 ul
Ch
FACS, ICC, IF

Cas9 antibody, 48796S, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 48796S, Cell Signaling Technology, Immunocytochemistry image

ICC

Supplier data
Cell Signaling Technology 34963S Cas9 antibody, 34963S, Cell Signaling Technology.

Mouse Mono [34963S], 100 ul
Ch
FACS, ICC, IF

Cas9 antibody, 34963S, Cell Signaling Technology, Flow Cytometry image

FACS,

Cas9 antibody, 34963S, Cell Signaling Technology, Immunocytochemistry image

ICC

Western Blot

Supplier data
Immuquest IQ651 Cas9 antibody, IQ651, Immuquest. Legend: Western blot - Anti CRISPR/Cas9 Antibody [7A9-3A3]

Mouse Mono [7A9-3A3], 100 ul
Bac
DB, IF, IP, WB

Cas9 antibody, IQ651, Immuquest, Dot Blot image

DB, IF, IP

Supplier data
Novus Biologicals NBP2-52398

Cas9 antibody, NBP2-52398, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody (6G12) - C-terminus [NBP2-52398] - CRISPR-Cas9 Antibody (6G12) - C-Terminus [NBP2-52398] - Control HeLa cells (un-transfected) and HeLa cells expressing Flag-tagged S. pyogenes's CRISPR-Cas9 under the control of PTight (Tet-ON) promoter. Samples were treated for 24 hours with 1ug/uL of Doxycyclin and lysed under native conditions. 30 ug of the whole cell lysate from each sample type per lane was separated by 7.5% SDS-PAGE. Nitrocellulose membrane was incubated

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

ChIP,

ICC,

Cas9 antibody, NBP2-52398, Novus Biologicals, Immunofluorescence image

IF,

Supplier data
Novus Biologicals NBP2-52718 Cas9 antibody, NBP2-52718, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody [NBP2-52718] - Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Cas9 antibody, NBP2-52718, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody [NBP2-52718] - Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15ug per lane. Primary Antibody cas9 used at 1ug/mL overnight at 4C. Secondary Antibody: goat anti-rabbit HRP at 1:40,000 for 30 min at room temp. Expect: 158kDa.

Rabbit Poly, 100 ug
Bac
ELISA, IF, WB

ELISA,

Cas9 antibody, NBP2-52718, Novus Biologicals, Immunofluorescence image

Supplier data
Novus Biologicals NBP2-67000 Cas9 antibody, NBP2-67000, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody (JM11-55) [NBP2-67000] - Analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1/500 dilution.

Cas9 antibody, NBP2-67000, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody (JM11-55) [NBP2-67000] - Analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1/500 dilution.

Rabbit Mono [JM11-55], 100 ul
Bac
FACS, ICC, IF, IHC-F, IHC-P, WB

FACS, ICC, IF, IHC-F, IHC-P

Supplier data
Novus Biologicals NBP2-81124

Cas9 antibody, NBP2-81124, Novus Biologicals. Western Blot: CRISPR-Cas9 Antibody (6H4) [NBP2-81124] - Total protein from mock and Cas9 transfected 293 cells, HeLa and 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Cas9 in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Theoretical molecular weight: 158.4 kDa.

Mouse Mono [6H4], 100 ug
Mo
ICC, IF, IP, WB

ICC, IF, IP

Supplier data
Invitrogen Antibodies MA1-202

Cas9 antibody, MA1-202, Invitrogen Antibodies. Western blot analysis of Cas9 was performed by loading indicated amounts of Cas9 nuclease and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. Cas9 was detected at 160 kDa using a Cas9 monoclonal antibody (Product # MA1-202) at a di

Mouse Mono [10C11-A12], 100 ug
Hu
FACS, IF, WB

FACS,

Cas9 antibody, MA1-202, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies MA5-23519 Cas9 antibody, MA5-23519, Invitrogen Antibodies. Western Blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9. Figure 2 shows the result of the WB on protein extracts from transfected (lane 2 and 4) and untransfected (lane 1 and 3) cells using a CRISPR/Cas9 monoclonal antibody (Product # MA5-23519) (lane 1 and 2) or an anti-myc antibody (lane 3 and 4).

Cas9 antibody, MA5-23519, Invitrogen Antibodies. Western Blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9. Figure 2 shows the result of the WB on protein extracts from transfected (lane 2 and 4) and untransfected (lane 1 and 3) cells using a CRISPR/Cas9 monoclonal antibody (Product # MA5-23519) (lane 1 and 2) or an anti-myc antibody (lane 3 and 4).

Mouse Mono [7A9], 50 ug
Hu
ICC, IF, IP, WB

ICC,

Cas9 antibody, MA5-23519, Invitrogen Antibodies, Immunofluorescence image

IF,

Supplier data
Invitrogen Antibodies MA1-201

Cas9 antibody, MA1-201, Invitrogen Antibodies. Western blot analysis of Cas9 was performed by loading indicated amounts of Cas9 nuclease and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. Cas9 was detected at 160 kDa using a Cas9 monoclonal antibody (Product # MA1-201) at a di

Mouse Mono [7A9-3A3], 100 ug
Hu
IF, WB

Cas9 antibody, MA1-201, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Cell Signaling Technology 19526T Cas9 antibody, 19526T, Cell Signaling Technology.

Rabbit Mono [19526T], 20 ul
Ch
AP, FACS, ICC, IF, IHC-P, WB

AP,

FACS,

ICC,

Cas9 antibody, 19526T, Cell Signaling Technology, Immunofluorescence image

IF,

IHC-P

Supplier data
Cell Signaling Technology 14697S Cas9 antibody, 14697S, Cell Signaling Technology.

Mouse Mono [14697S], 100 ul
Ch
FACS, ICC, IF, IHC-P, WB

FACS,

ICC,

Cas9 antibody, 14697S, Cell Signaling Technology, Immunofluorescence image

IF,

IHC-P

Supplier data
Cell Signaling Technology 14697T Cas9 antibody, 14697T, Cell Signaling Technology.

Mouse Mono [14697T], 20 ul
Ch
AP, FACS, ICC, IF, IHC-P, WB

AP,

FACS,

ICC,

Cas9 antibody, 14697T, Cell Signaling Technology, Immunofluorescence image

IF,

IHC-P

Supplier data
Cell Signaling Technology 19526S Cas9 antibody, 19526S, Cell Signaling Technology.

Rabbit Mono [19526S], 100 ul
Ch
FACS, ICC, IF, IHC-P, WB

FACS,

ICC,

Cas9 antibody, 19526S, Cell Signaling Technology, Immunofluorescence image

IF,

IHC-P

Supplier data
Cell Signaling Technology 65832S Cas9 antibody, 65832S, Cell Signaling Technology.

Rabbit Mono [65832S], 100 ul
Ch
FACS, ICC, IF, WB

FACS,

ICC,

Cas9 antibody, 65832S, Cell Signaling Technology, Immunofluorescence image

Supplier data
Cell Signaling Technology 48989S Cas9 antibody, 48989S, Cell Signaling Technology.

Mouse Mono [48989S], 100 ul
Ch
ICC, IF, IP, WB

ICC,

Cas9 antibody, 48989S, Cell Signaling Technology, Immunofluorescence image

IF,

Supplier data
Cell Signaling Technology 51610S Cas9 antibody, 51610S, Cell Signaling Technology.

Rabbit Mono [51610S], 100 ul
Ch
AP, FACS, ICC, IF, WB

AP,

FACS,

ICC,

Cas9 antibody, 51610S, Cell Signaling Technology, Immunofluorescence image

Immunocytochemistry

Supplier data
Novus Biologicals NBP2-52398

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

ChIP,

IF,

IP,

Supplier data
Cell Signaling Technology 19526T Cas9 antibody, 19526T, Cell Signaling Technology.

Rabbit Mono [19526T], 20 ul
Ch
AP, FACS, ICC, IF, IHC-P, WB

AP,

FACS,

IF,

IHC-P,

Supplier data
Cell Signaling Technology 14697S Cas9 antibody, 14697S, Cell Signaling Technology.

Mouse Mono [14697S], 100 ul
Ch
FACS, ICC, IF, IHC-P, WB

FACS,

IF,

IHC-P,

Supplier data
Cell Signaling Technology 14697T Cas9 antibody, 14697T, Cell Signaling Technology.

Mouse Mono [14697T], 20 ul
Ch
AP, FACS, ICC, IF, IHC-P, WB

AP,

FACS,

IF,

IHC-P,

Supplier data
Cell Signaling Technology 19526S Cas9 antibody, 19526S, Cell Signaling Technology.

Rabbit Mono [19526S], 100 ul
Ch
FACS, ICC, IF, IHC-P, WB

FACS,

IF,

IHC-P,

Supplier data
Cell Signaling Technology 65832S Cas9 antibody, 65832S, Cell Signaling Technology.

Rabbit Mono [65832S], 100 ul
Ch
FACS, ICC, IF, WB

FACS,

IF,

Supplier data
Cell Signaling Technology 48989S Cas9 antibody, 48989S, Cell Signaling Technology.

Mouse Mono [48989S], 100 ul
Ch
ICC, IF, IP, WB

IF,

IP,

Supplier data
Cell Signaling Technology 51610S Cas9 antibody, 51610S, Cell Signaling Technology.

Rabbit Mono [51610S], 100 ul
Ch
AP, FACS, ICC, IF, WB

AP,

FACS,

IF,

Supplier data
Cell Signaling Technology 48796S Cas9 antibody, 48796S, Cell Signaling Technology.

Mouse Mono [48796S], 100 ul
Ch
FACS, ICC, IF

FACS,

Cas9 antibody, 48796S, Cell Signaling Technology, Immunofluorescence image

Supplier data
Cell Signaling Technology 34963S Cas9 antibody, 34963S, Cell Signaling Technology.

Mouse Mono [34963S], 100 ul
Ch
FACS, ICC, IF

FACS,

Cas9 antibody, 34963S, Cell Signaling Technology, Immunofluorescence image

Immunoprecipitation

Supplier data
Novus Biologicals NBP2-52398

Mouse Mono [6G12], 100 ul
Bac
ChIP, ICC, IF, IP, WB

ChIP,

ICC,

IF,

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

43 matching Cas9 antibodies for Immunofluorescence from 10 suppliers

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Cas9 information

Aliases

1 Applications, 5 Species currently selected

43 matching Cas9 antibodies for Immunofluorescence after applying filters

Immunofluorescence - Use Filter to switch application

Western Blot

Immunocytochemistry

Immunoprecipitation

All None
	WB	IF	ICC	FACS	IHC-P	IP	IHC-F	AP	ELISA	ChIP	DB
Bacteria	61	45	43	2	5	4	4		2	2	1
Human	11	4	2	1	1	3	1
Chicken	11	10	9	11	5	1		3
Mouse	2	2	2			2				1
Yeast	1