SMAD Family Member 7 Antibodies for Flow Cytometry

4 matching SMAD Family Member 7 antibodies for Flow Cytometry after applying filters

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Supplier data
Invitrogen Antibodies 42-0400

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies. Flow cytometry analysis of SMAD7 was done on Jurkat cells treated with TNF alpha (50ng/mL, 20 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with SMAD7 Rabbit Polyclonal Antibody (420400, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells

Rabbit Poly, 100 ug
Hu, Mo, Rt
FACS, ICC, IF, IHC-F, IHC-P, WB

ICC,

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Immunofluorescence image

IF,

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Immunohistochemistry frozen image

IHC-F,

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Immunohistochemistry paraffin image

IHC-P,

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 701940

SMAD Family Member 7 antibody, 701940, Invitrogen Antibodies. Flow Cytometry analysis of SMAD7 [c-term] was performed on A549 cells labeled with ABfinity™ Anti-SMAD7 [c-term] Recombinant Rabbit Monoclonal Antibody (Product# 701940, 2-4 ug/ 1M cells) or with Rabbit isotype control and detected with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, (Alexa Fluor® 488 conjugate, Product # A27034, 0.4 ug/ml, 1:2500) as represented by the red and yellow histograms respectively. The purple histogram represents unstained control cells and

Rabbit Mono [17H18L23], 100 ug
Hu
FACS, ICC, IF, WB

ICC,

SMAD Family Member 7 antibody, 701940, Invitrogen Antibodies, Immunofluorescence image

IF,

Western Blot

Supplier data
Invitrogen Antibodies 42-0400

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies. Knockdown of SMAD7 was achieved by transfecting HeLa cells with SMAD7 specific siRNAs (Silencer® select Product # s8412). Western blot analysis (Fig a) was performed using whole cell extracts from the SMAD7 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and un-transfected cells (lane 1). The blots were probed with Anti-SMAD7 Rabbit Polyclonal Antibody (Product # 42-0400, 2µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HR

Rabbit Poly, 100 ug
Hu, Mo, Rt
FACS, ICC, IF, IHC-F, IHC-P, WB

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Flow Cytometry image

FACS, ICC, SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Immunofluorescence image

IF,

IHC-F,

IHC-P

Supplier data
Invitrogen Antibodies 701940 SMAD Family Member 7 antibody, 701940, Invitrogen Antibodies. Western blot analysis was performed on whole cell extracts (50

SMAD Family Member 7 antibody, 701940, Invitrogen Antibodies. Western blot analysis was performed on whole cell extracts (50

Rabbit Mono [17H18L23], 100 ug
Hu
FACS, ICC, IF, WB

SMAD Family Member 7 antibody, 701940, Invitrogen Antibodies, Flow Cytometry image

FACS, ICC, SMAD Family Member 7 antibody, 701940, Invitrogen Antibodies, Immunofluorescence image

Immunohistochemistry paraffin

Supplier data
Invitrogen Antibodies 42-0400

SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies. Immunohistochemistry analysis of SMAD7 showing staining in the cytoplasm and nucleus of paraffin-embedded rat rectum tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SMAD7 Rabbit Polyclonal Antibody

Rabbit Poly, 100 ug
Hu, Mo, Rt
FACS, ICC, IF, IHC-F, IHC-P, WB

FACS, ICC, SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Immunofluorescence image

IF,

IHC-F,

Immunohistochemistry frozen

Supplier data
Invitrogen Antibodies 42-0400

Rabbit Poly, 100 ug
Hu, Mo, Rt
FACS, ICC, IF, IHC-F, IHC-P, WB

FACS, ICC, SMAD Family Member 7 antibody, 42-0400, Invitrogen Antibodies, Immunofluorescence image

IF,

IHC-P,

Immunofluorescence

SMAD Family Member 7 antibodies are often published with	Associated citations
SMAD2	38.5%
Cadherin 1	19.2%
SMAD Specific E3 Ubiquitin Protein Ligase 2	11.5%
Fibronectin 1	11.5%
Ubiquitin B	11.5%
Caspase 3	11.5%
SMAD Family Member 3	11.5%
IL-4	7.7%
Synaptotagmin 1	7.7%
p65	7.7%

SMAD Family Member 7 antibodies are often published with

Associated citations

SMAD2

38.5%

Cadherin 1

19.2%

SMAD Specific E3 Ubiquitin Protein Ligase 2

11.5%

11.5%

11.5%

11.5%

11.5%

7.7%

7.7%

7.7%

All None
	WB	IHC-P	IHC-F	IF	ELISA	ICC	AP	FACS	IP	Pep-ELISA	ChIP
Human	171	107	84	75	53	43	10	6	5	3	1
Rat	101	57	37	50	24	36		5	3	3	1
Mouse	101	54	37	50	27	36		5	4	3	1
Sheep	21	22	21								1
Bovine	8	3	2		1				1
Primate	7	3	1	1
Guinea Pig	6	1			1
Porcine	3	1	1	6	1	6			1
Canine	4	1	1
Rabbit	3	1		1	1				1
Bat	1
Chicken	1	1
Xenopus	1	1
Hamster	1	1
Equine	1	1
Fish	1
Bacteria	1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

4 matching SMAD Family Member 7 antibodies for Flow Cytometry from 12 suppliers

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SMAD Family Member 7 information

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1 Applications, 17 Species currently selected

4 matching SMAD Family Member 7 antibodies for Flow Cytometry after applying filters

Flow Cytometry - Use Filter to switch application

Western Blot

Immunohistochemistry paraffin

Immunohistochemistry frozen

Immunofluorescence

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