5-hmC Polyclonal Antibodies

7 5-hmC polyclonal antibodies from 2 antibody suppliers Click each 5-hmC polyclonal antibody that interests you to view the full 5-hmC polyclonal antibody datasheet.

Immunoprecipitation - Use Filter to switch application

Supplier data
Novus Biologicals NBP2-59184

5-hmC antibody, NBP2-59184, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59184] - hMeDIP (hydroxymethylated DNA IP) was performed using the antibody against 5-hydroxymethylcytosine. DNA from mouse ES cells was prepared and sonicated to obtain DNA fragments of 300-500 bp. One ug of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls. hMeDIP was performed with 3.5 ug of the rabbit 5-hmC antibody and the IP'd DNA was analysed by qPCR using primers specific for the

Rabbit Poly, 50 ug
Hu, Mo, Xe, Zf
DB, ELISA, IP

5-hmC antibody, NBP2-59184, Novus Biologicals, Dot Blot image

DB,

5-hmC antibody, NBP2-59184, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA

Supplier data
Novus Biologicals NBP2-43569 5-hmC antibody, NBP2-43569, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-43569] - Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

5-hmC antibody, NBP2-43569, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-43569] - Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Rabbit Poly, 100 ul

DB, IP

5-hmC antibody, NBP2-43569, Novus Biologicals, Dot Blot image

Supplier data
Novus Biologicals NBP2-59182

5-hmC antibody, NBP2-59182, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59182] - An hydroxymethylated DNA IP (hMeDIP) was performed using the rabbit polyclonal antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from rabbit were used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cell DNA was spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP'd materia

Rabbit Poly, 100 ul
Hu, Mo
DB, ELISA, IP

DB,

5-hmC antibody, NBP2-59182, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA

Supplier data
Invitrogen Antibodies PA5-40097

5-hmC antibody, PA5-40097, Invitrogen Antibodies. hMeDIP (hydroxymethylated DNA IP) was performed using a 5-hmC/5-hydroxymethylcytosine polyclonal antibody (Product # PA5-40097). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One µg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls. hMeDIP was performed with 3.5 µg of the rabbit 5-hmC antibody and th

Rabbit Poly, 50 ug
Hu, Mo
AP, ELISA, IP

AP,

5-hmC antibody, PA5-40097, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA

Dot Blot

Supplier data
Novus Biologicals NBP2-59184

Rabbit Poly, 50 ug
Hu, Mo, Xe, Zf
DB, ELISA, IP

ELISA,

5-hmC antibody, NBP2-59184, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-43569

5-hmC antibody, NBP2-43569, Novus Biologicals. Dot Blot: 5-Hydroxymethylcytosine Antibody [NBP2-43569] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:50000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.

Rabbit Poly, 100 ul

DB, IP

5-hmC antibody, NBP2-43569, Novus Biologicals, Immunoprecipitation image

Enzyme Linked Immunosorbent Assay

Supplier data
Novus Biologicals NBP2-59184

5-hmC antibody, NBP2-59184, Novus Biologicals. ELISA: 5-Hydroxymethylcytosine Antibody [NBP2-59184] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC , crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.

Rabbit Poly, 50 ug
Hu, Mo, Xe, Zf
DB, ELISA, IP

DB,

Supplier data
Novus Biologicals NBP2-59182

5-hmC antibody, NBP2-59182, Novus Biologicals. ELISA: 5-Hydroxymethylcytosine Antibody [NBP2-59182] - Determination of the 5-hmC rabbit polyclonal antibody titer. To determine the titer, an ELISA was performed using a serial dilution of the rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500.

Rabbit Poly, 100 ul
Hu, Mo
DB, ELISA, IP

DB,

5-hmC antibody, NBP2-59182, Novus Biologicals, Immunoprecipitation image

Supplier data
Invitrogen Antibodies PA5-40097 5-hmC antibody, PA5-40097, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-5-hmC antibody (Product # PA5-40097), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.

5-hmC antibody, PA5-40097, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-5-hmC antibody (Product # PA5-40097), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.

Rabbit Poly, 50 ug
Hu, Mo
AP, ELISA, IP

AP,

5-hmC antibody, PA5-40097, Invitrogen Antibodies, Immunoprecipitation image

Western Blot

Supplier data
Novus Biologicals NBP2-60700 5-hmC antibody, NBP2-60700, Novus Biologicals. Western Blot: HMCES Antibody [NBP2-60700] - Detection of human HMCES by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-HMCES antibody NBP2-60700 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Poly, 100 ul
Hu
WB

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

7 matching 5-hmC polyclonal antibodies from 2 suppliers

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