5-hmC Antibodies for Immunoprecipitation

2 matching 5-hmC antibodies for Immunoprecipitation after applying filters

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Supplier data
Novus Biologicals NBP2-61469 5-hmC antibody, NBP2-61469, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the co

5-hmC antibody, NBP2-61469, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the co

Rabbit Mono [RM236], 50 ug
Vert
AP, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

AP,

5-hmC antibody, NBP2-61469, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

5-hmC antibody, NBP2-61469, Novus Biologicals, Flow Cytometry image

FACS,

5-hmC antibody, NBP2-61469, Novus Biologicals, Immunocytochemistry image

ICC,

5-hmC antibody, NBP2-61469, Novus Biologicals, Immunofluorescence image

IF,

5-hmC antibody, NBP2-61469, Novus Biologicals, Immunohistochemistry frozen image

IHC-F,

5-hmC antibody, NBP2-61469, Novus Biologicals, Immunohistochemistry paraffin image

IHC-P

Supplier data
Novus Biologicals NBP2-59184

5-hmC antibody, NBP2-59184, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59184] - hMeDIP (hydroxymethylated DNA IP) was performed using the antibody against 5-hydroxymethylcytosine. DNA from mouse ES cells was prepared and sonicated to obtain DNA fragments of 300-500 bp. One ug of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls. hMeDIP was performed with 3.5 ug of the rabbit 5-hmC antibody and the IP'd DNA was analysed by qPCR using primers specific for the

Rabbit Poly, 50 ug
Hu, Mo, Xe, Zf
DB, ELISA, IP

5-hmC antibody, NBP2-59184, Novus Biologicals, Dot Blot image

DB,

5-hmC antibody, NBP2-59184, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA

Supplier data
Novus Biologicals NBP2-59177

5-hmC antibody, NBP2-59177, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15200200) [NBP2-59177] - 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - An hydroxymethylated DNA IP (hMeDIP) was performed using the antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from mouse was used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cells DNA were spiked with non-methylated, methy

Mouse Mono [C.15200200], 100 ug
Hu, Mo
DB, ELISA, IP

5-hmC antibody, NBP2-59177, Novus Biologicals, Dot Blot image

DB,

5-hmC antibody, NBP2-59177, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA

Supplier data
Novus Biologicals NBP2-43568 5-hmC antibody, NBP2-43568, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody (513) [NBP2-43568] - Analysis of Human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Hydroxymethylcytosine/ 5-hmC antibody [513] at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.

5-hmC antibody, NBP2-43568, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody (513) [NBP2-43568] - Analysis of Human genomic DNA (500 ng) mixed with methylated cytosine standard kit, using 5-Hydroxymethylcytosine/ 5-hmC antibody [513] at 1:500 dilution. Analysized with semi-quantitative PCR and Immunprecipitated DNA was plotted as % of input DNA.

Mouse Mono [513], 100 ul
Hu
DB, IP

5-hmC antibody, NBP2-43568, Novus Biologicals, Dot Blot image

Supplier data
Novus Biologicals NBP2-59182

5-hmC antibody, NBP2-59182, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59182] - An hydroxymethylated DNA IP (hMeDIP) was performed using the rabbit polyclonal antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from rabbit were used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cell DNA was spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP'd materia

Rabbit Poly, 100 ul
Hu, Mo
DB, ELISA, IP

DB,

5-hmC antibody, NBP2-59182, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA

Supplier data
Invitrogen Antibodies MA5-24695

5-hmC antibody, MA5-24695, Invitrogen Antibodies. hMeDIP was performed using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 µg of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.

Rabbit Mono [RM236], 50 ug
Hu
DB, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Dot Blot image

DB,

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Flow Cytometry image

FACS,

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Immunocytochemistry image

ICC, IF,

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Immunohistochemistry frozen image

IHC-F,

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Immunohistochemistry paraffin image

IHC-P

Supplier data
Invitrogen Antibodies MA5-23525

5-hmC antibody, MA5-23525, Invitrogen Antibodies. Immunoprecipitation of 5-HydroxymethylCytosine in Methylated DNA immunoprecipitation of Human genomic DNA (500 ng) mixed with methylated cytosine standard kit using a 5-HydroxymethylCytosine monoclonal antibody (Product # MA5-23525) at dilution of 1:500. Samples were analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Mouse Mono [3I7], 100 ul
chem
DB, IHC-F, IHC-P, IP

5-hmC antibody, MA5-23525, Invitrogen Antibodies, Dot Blot image

DB,

5-hmC antibody, MA5-23525, Invitrogen Antibodies, Immunohistochemistry frozen image

IHC-F,

5-hmC antibody, MA5-23525, Invitrogen Antibodies, Immunohistochemistry paraffin image

IHC-P

Supplier data
Invitrogen Antibodies PA5-40097

5-hmC antibody, PA5-40097, Invitrogen Antibodies. hMeDIP (hydroxymethylated DNA IP) was performed using a 5-hmC/5-hydroxymethylcytosine polyclonal antibody (Product # PA5-40097). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One µg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) controls. hMeDIP was performed with 3.5 µg of the rabbit 5-hmC antibody and th

Rabbit Poly, 50 ug
Hu, Mo
AP, ELISA, IP

AP,

5-hmC antibody, PA5-40097, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA

Show/hide more from Novus Biologicals

Supplier data
Novus Biologicals NBP2-59178

5-hmC antibody, NBP2-59178, Novus Biologicals. Methylated DNA Immunoprecipitation: 5-hmC Antibody (C.15220001) [NBP2-59178] - Hydroxymethylated DNA IP (hMeDIP) assays were performed using the antibody against 5-hydroxymethylcytosine, 5-hmC, 5-mC and cytosine DNA standards and Rat IgG. DNA fragments are 300-500 bp. 1 ug of mouse ES cells DNA was spiked with 0.025 ng of each DNA standard. The IP'd material has been analyzed by qPCR using the primer pairs specific to the control sequences. The obtained results are as follows: - hMeDIP on unmeth

Rat Mono [C.15220001], 100 ug
Dr, Hu, Mo
DB, ELISA, IP

DB,

5-hmC antibody, NBP2-59178, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA

Dot Blot

Supplier data
Novus Biologicals NBP2-59184

Rabbit Poly, 50 ug
Hu, Mo, Xe, Zf
DB, ELISA, IP

ELISA,

5-hmC antibody, NBP2-59184, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-59177 5-hmC antibody, NBP2-59177, Novus Biologicals. Dot Blot: 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - Dot Blot analysis of the antibody with the C, mC and hmC PCR controls 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the antibody (dilution 1:500). The membranes were exposed for 30 seconds.

5-hmC antibody, NBP2-59177, Novus Biologicals. Dot Blot: 5-Hydroxymethylcytosine Antibody (C.15200200) [NBP2-59177] - Dot Blot analysis of the antibody with the C, mC and hmC PCR controls 200 to 2 ng (equivalent of 10 to 0.1 pmol of C-bases) of the hmC (1), mC (2) and C (3) PCR controls from the antibody (dilution 1:500). The membranes were exposed for 30 seconds.

Mouse Mono [C.15200200], 100 ug
Hu, Mo
DB, ELISA, IP

ELISA,

5-hmC antibody, NBP2-59177, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-43568

5-hmC antibody, NBP2-43568, Novus Biologicals. Dot Blot: 5-hmC Antibody (513) [NBP2-43568] - Analysis of anti-5-hmC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.

Mouse Mono [513], 100 ul
Hu
DB, IP

5-hmC antibody, NBP2-43568, Novus Biologicals, Immunoprecipitation image

Supplier data
Invitrogen Antibodies MA5-24695 5-hmC antibody, MA5-24695, Invitrogen Antibodies. Dot blot of double stranded DNA using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA.

5-hmC antibody, MA5-24695, Invitrogen Antibodies. Dot blot of double stranded DNA using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695). The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA.

Rabbit Mono [RM236], 50 ug
Hu
DB, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

ELISA,

FACS,

ICC, IF,

IHC-F,

IHC-P,

5-hmC antibody, MA5-24695, Invitrogen Antibodies, Immunoprecipitation image

Supplier data
Invitrogen Antibodies MA5-23525

5-hmC antibody, MA5-23525, Invitrogen Antibodies. Dot blot analysis of 5-HydroxymethylCytosine using a 5-HydroxymethylCytosine monoclonal antibody (Product # MA5-23525) with synthetic DNA controls. DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged nylon membrane and blotted with primary antibody at a dilution of 1:500. (A) Unmethylated DNA fragment (B) DNA fragment containing 5-methylcytosine (C) DNA fragment containing 5-hydroxymethylcytosine (D) DNA fragment containing 5-formylcytosine (E) DNA fragment containin

Mouse Mono [3I7], 100 ul
chem
DB, IHC-F, IHC-P, IP

IHC-F,

IHC-P,

5-hmC antibody, MA5-23525, Invitrogen Antibodies, Immunoprecipitation image

Enzyme Linked Immunosorbent Assay

Supplier data
Novus Biologicals NBP2-61469

5-hmC antibody, NBP2-61469, Novus Biologicals. ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - ELISA of single stranded DNA using Anti-5-hmC antibody (NBP2-61469). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61469 was used as the primary antibody, and an alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.

Rabbit Mono [RM236], 50 ug
Vert
AP, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

AP,

FACS,

ICC,

IF,

IHC-F,

IHC-P,

5-hmC antibody, NBP2-61469, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-59184

5-hmC antibody, NBP2-59184, Novus Biologicals. ELISA: 5-Hydroxymethylcytosine Antibody [NBP2-59184] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC , crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.

Rabbit Poly, 50 ug
Hu, Mo, Xe, Zf
DB, ELISA, IP

DB,

Supplier data
Novus Biologicals NBP2-59177 5-hmC antibody, NBP2-59177, Novus Biologicals. ELISA: 5-hmC Antibody (C.15200200) [NBP2-59177] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.

5-hmC antibody, NBP2-59177, Novus Biologicals. ELISA: 5-hmC Antibody (C.15200200) [NBP2-59177] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was KHL coupled to 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.

Mouse Mono [C.15200200], 100 ug
Hu, Mo
DB, ELISA, IP

DB,

Supplier data
Novus Biologicals NBP2-59182

5-hmC antibody, NBP2-59182, Novus Biologicals. ELISA: 5-Hydroxymethylcytosine Antibody [NBP2-59182] - Determination of the 5-hmC rabbit polyclonal antibody titer. To determine the titer, an ELISA was performed using a serial dilution of the rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500.

Rabbit Poly, 100 ul
Hu, Mo
DB, ELISA, IP

DB,

5-hmC antibody, NBP2-59182, Novus Biologicals, Immunoprecipitation image

Supplier data
Novus Biologicals NBP2-59178

5-hmC antibody, NBP2-59178, Novus Biologicals. ELISA: 5-Hydroxymethylcytosine Antibody (C.15220001) [NBP2-59178] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against 5-hmC in antigen coated wells. The antigen used was a 5-hmC base coupled to KHL. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.

Rat Mono [C.15220001], 100 ug
Dr, Hu, Mo
DB, ELISA, IP

DB,

5-hmC antibody, NBP2-59178, Novus Biologicals, Immunoprecipitation image

Supplier data
Invitrogen Antibodies MA5-24695 5-hmC antibody, MA5-24695, Invitrogen Antibodies. Direct ELISA of Mouse brain genomic DNA using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. 1

5-hmC antibody, MA5-24695, Invitrogen Antibodies. Direct ELISA of Mouse brain genomic DNA using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. 1

Rabbit Mono [RM236], 50 ug
Hu
DB, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

DB,

FACS,

ICC, IF,

IHC-F,

IHC-P,

Supplier data
Invitrogen Antibodies PA5-40097 5-hmC antibody, PA5-40097, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-5-hmC antibody (Product # PA5-40097), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.

5-hmC antibody, PA5-40097, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-5-hmC antibody (Product # PA5-40097), crude serum and flow through, in antigen coated wells. The antigen used was the 5-hmC base coupled to BSA. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,800.

Rabbit Poly, 50 ug
Hu, Mo
AP, ELISA, IP

AP,

5-hmC antibody, PA5-40097, Invitrogen Antibodies, Immunoprecipitation image

Immunohistochemistry frozen

Supplier data
Novus Biologicals NBP2-61469 5-hmC antibody, NBP2-61469, Novus Biologicals. Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using Rabbit monoclonal Anti-5-hmC (NBP2-61469) antibody.

5-hmC antibody, NBP2-61469, Novus Biologicals. Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using Rabbit monoclonal Anti-5-hmC (NBP2-61469) antibody.

Rabbit Mono [RM236], 50 ug
Vert
AP, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

AP,

ELISA,

FACS,

ICC,

IF,

IHC-P,

Supplier data
Invitrogen Antibodies MA5-24695 5-hmC antibody, MA5-24695, Invitrogen Antibodies. Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695).

5-hmC antibody, MA5-24695, Invitrogen Antibodies. Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using 5-Hydroxymethylcytosine Antibody (Product # MA5-24695).

Rabbit Mono [RM236], 50 ug
Hu
DB, ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

DB,

ELISA,

FACS,

ICC, IF,

IHC-P,

Supplier data
Invitrogen Antibodies MA5-23525 5-hmC antibody, MA5-23525, Invitrogen Antibodies. Immunohistochemical analysis of 5-HydroxymethylCytosine in paraffin-embedded mouse testis tissue using a 5-HydroxymethylCytosine monoclonal antibody (Product # MA5-23525) at a dilution of 1:100.

5-hmC antibody, MA5-23525, Invitrogen Antibodies. Immunohistochemical analysis of 5-HydroxymethylCytosine in paraffin-embedded mouse testis tissue using a 5-HydroxymethylCytosine monoclonal antibody (Product # MA5-23525) at a dilution of 1:100.

Mouse Mono [3I7], 100 ul
chem
DB, IHC-F, IHC-P, IP

DB,

IHC-P,

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

2 matching 5-hmC antibodies for Immunoprecipitation from 6 suppliers

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5-hmC information

Aliases

1 Applications, 8 Species currently selected

2 matching 5-hmC antibodies for Immunoprecipitation after applying filters

Immunoprecipitation - Use Filter to switch application

Dot Blot

Enzyme Linked Immunosorbent Assay

Immunohistochemistry frozen

Immunohistochemistry paraffin

All None
	IP	ELISA	DB	IF	AP	IHC-F	WB	ICC	FACS	IHC-P
Human	7	6	6	1	1	1	1	1	1	1
Mouse	5	5	4		1
Xenopus	1	1	1
Zebrafish	1	1	1
Drosophila	1	1	1
Vertebrates	1	1		1	1	1		1	1	1
Chemical	1		1			1				1
Chicken		1	1	1				1