CODEX® Immunofluorescence
Leinco Technologies is the leading supplier of CODEX® reagents, providing researchers with CODEX® barcoded antibodies, CODEX® validated antibodies, and custom DNA-barcoded antibody services.
Antibodies
Antibody Services
Ready” Antibodies
Technology
CODEX® Barcoded Antibodies
Leinco manufactures and inventories CODEX® barcoded antibodies and complementary fluorophore-conjugated oligonucleotides. These “Ready To Stain” DNA barcoded antibodies minimize user optimization for the CODEX® high multiplex imaging system.
‘Ready to Stain’ DNA barcoded antibodies are validated according to tissue preparation, such as fresh frozen (FF) or fresh frozen paraffin-embedded (FFPE), and by tissue screened, such as liver, spleen, tonsil, brain, etc. Leinco’s DNA barcoded antibodies for CODEX® have undergone varying levels of validation and quality testing to assure conjugations are free of unconjugated antibodies and DNA barcodes.
Custom DNA Barcoded Antibody Services
Undoubtedly, Leinco is bridging the gap to simplify the development and manufacturing process for CODEX® users. Custom services includes the post-purification modification of oligonucleotide barcodes to custom CODEX® antibodies of your choosing. Mix and match with three CODEX® reporters, each with a distinct dye. The 4 step-process is as followed:
Step 1
Choose purified antibodies screened and validated for use in CODEX®.
Step 2
Choose the barcode.
Step 3
Choose a matched Fluorescence reporter for your conjugated antibodies Barcoded reporter molecules include Alexa Fluor™ 488, Alexa Fluor™ 750, Atto 550, and Cy5.
Step 4
Receive Antibodies conjugated to unique oligonucleotide barcodes paired with reporter molecules conjugated to complementary barcodes.
Validated “CODEX® Ready” Antibodies
Leinco purified (CODEX® Ready) antibodies are suitable for oligonucleotide conjugation, formulated in conjugate ready buffers to minimize the antibody preparation needed when performing a CODEX® barcode conjugate.
Featured Proteins and Clones
View all CODEX® validated antibodies >>>
The CODEX® Technology
Overcoming challenges of traditional multiplexed immunofluorescence staining has now become easier with the CODEX® ultrahigh multiplexed imaging system and assay reagents. This innovative, highly scalable technology targets subsets of an antibody panel through repetitive imaging cycles while revealing over 40 biomarkers in a single tissue section. The CODEX® technology was pioneered in Dr. Nolan’s lab at Stanford and now patented by Akoya Biosciences.
The Benefits
- A comprehensive end-to-end solution containing the fluidics platform, assay reagents, and bioinformatics
- Benchtop footprint that integrates with an existing fluorescence microscope
- The system is scalable and capable of imaging up to 50 biomarkers per run
- Preserves the sample for Region Of Interest (ROI) analysis and H&E staining
- FF and FFPE tissue compatible
