Tumor Necrosis Factor Antibodies for Flow Cytometry

725 matching Tumor Necrosis Factor antibodies for Flow Cytometry after applying filters

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Supplier data
Origene AM26711RP-N Tumor Necrosis Factor antibody, AM26711RP-N, Origene.

Mouse Mono [Clone Mab11], 100 Test
Hu, Po, Pr
FACS

Supplier data
Origene AM26711AC-N Tumor Necrosis Factor antibody, AM26711AC-N, Origene.

Mouse Mono [Clone Mab11], 100 Test
Hu, Po, Pr
FACS

Supplier data
Sino Biological 10602-R101-F Tumor Necrosis Factor antibody, 10602-R101-F, Sino Biological. FACS

Rabbit Mono [101], 100 Test
Hu
FACS

Supplier data
Sino Biological 10602-MM01-F Tumor Necrosis Factor antibody, 10602-MM01-F, Sino Biological.

Mouse Mono [1], 100 Test
Hu
FACS

Supplier data
Sino Biological 10602-MM01 Tumor Necrosis Factor antibody, 10602-MM01, Sino Biological.

Mouse Mono [9A7A5G9], 100 ug
Hu
ELISA, FACS

ELISA

Supplier data
Sino Biological 10602-R101-P Tumor Necrosis Factor antibody, 10602-R101-P, Sino Biological.

Rabbit Mono [101], 100 Test
Hu
FACS

Supplier data
Sino Biological 10602-R101-A Tumor Necrosis Factor antibody, 10602-R101-A, Sino Biological.

Rabbit Mono [101], 100 Test
Hu
FACS

Supplier data
Bio-Rad (formerly AbD Serotec) MCA1487 Tumor Necrosis Factor antibody, MCA1487, Bio-Rad (formerly AbD Serotec) .

Rat Mono [MP6-XT3], 500 ug
Mo
ELISA, FACS, WB

Tumor Necrosis Factor antibody, MCA1487, Bio-Rad (formerly AbD Serotec) , Enzyme Linked Immunosorbent Assay image

ELISA,

Tumor Necrosis Factor antibody, MCA1487, Bio-Rad (formerly AbD Serotec) , Western Blot image

Show/hide more from Sino Biological

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Sino Biological 10602-R10N1 Tumor Necrosis Factor antibody, 10602-R10N1, Sino Biological.

Rabbit Mono [R101], 500 ug
Hu
FACS, Neu

Neu

Supplier data
Sino Biological 10602-R101 Tumor Necrosis Factor antibody, 10602-R101, Sino Biological.

Rabbit Mono [101], 100 ul
Hu
FACS

Supplier data
Bio-Rad (formerly AbD Serotec) MCA1488 Tumor Necrosis Factor antibody, MCA1488, Bio-Rad (formerly AbD Serotec) .

Rat Mono [MP6-XT22], 500 ug
Mo, Rt
ELISA, FACS, IHC-F

Tumor Necrosis Factor antibody, MCA1488, Bio-Rad (formerly AbD Serotec) , Enzyme Linked Immunosorbent Assay image

ELISA,

Tumor Necrosis Factor antibody, MCA1488, Bio-Rad (formerly AbD Serotec) , Immunohistochemistry frozen image

IHC-F

Supplier data
Bio-Rad (formerly AbD Serotec) MCA2334A488

Tumor Necrosis Factor antibody, MCA2334A488, Bio-Rad (formerly AbD Serotec) . <strong>Published customer image:</strong> <br>Phycoerythrin conjugated Mouse anti Bovine TNF alpha antibody, clone CC327 used for the evaluation of TNF-α expression by bovine PBMC following <em>M. bovis</em> infection.<br><strong>Image caption:</strong><br>Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural <em>M. bovis</em> infection. PBMC from naturally <em>M. bovis</em> infected cattle were cultured in the presence of either PPD-B or medium and the

Mouse Mono [CC327], 100 Test
Bv
FACS

Supplier data
Bio-Rad (formerly AbD Serotec) MCA1560PE

Tumor Necrosis Factor antibody, MCA1560PE, Bio-Rad (formerly AbD Serotec) . Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (<a href="https://www.bio-rad-antibodies.com/product/partnumber/MCA463F.html">MCA463F</a>) and PE conjugated Rat anti Human TNF alpha (<a href="https://www.bio-rad-antibodies.com/product/partnumber/MCA1560PE.html">MCA1560PE</a>). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (<a href="https://www.bio-rad-antibodies.com/product/partnumber/BUF077A.html">BUF077A</a>) for 6 hours wer

Rat Mono [MP9-20A4], 100 Test
Hu
FACS

Supplier data
Bio-Rad (formerly AbD Serotec) MCA1395F

Tumor Necrosis Factor antibody, MCA1395F, Bio-Rad (formerly AbD Serotec) . Figure A. Unstimulated cells were stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (<a href="https://www.bio-rad-antibodies.com/product/partnumber/MCA463A700.html">MCA463A700</a>) and FITC conjugated anti Human TNF alpha (<a href="https://www.bio-rad-antibodies.com/product/partnumber/MCA1395F.html">MCA1395F</a>). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (<a href="https://www.bio-rad-antibodies.com/product/partnumber/BUF077A.html">BUF077A</a>) fo

Mouse Mono [B-D9], 100 ug
Hu
FACS

Supplier data
Novus Biologicals NBP2-27224

Tumor Necrosis Factor antibody, NBP2-27224, Novus Biologicals. Flow Cytometry: TNF-alpha Antibody (6N1E7) - Azide Free [NBP2-27224] - Human PBMC were stimulated with PMA (10 ng/ml) and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with by intracellular staining. Fixed and permeabilized cells were incubated with 10 ul (0.5 ug) of TNF antibody (or ) for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.

Mouse Mono [6N1E7], 100 ug
Hu
Bl, FACS, WB

Tumor Necrosis Factor antibody, NBP2-27224, Novus Biologicals, Blocking image

Bl,

Tumor Necrosis Factor antibody, NBP2-27224, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-27223

Tumor Necrosis Factor antibody, NBP2-27223, Novus Biologicals. Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/ml) and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with by intracellular staining. Fixed and permeabilized cells were incubated with 10 ul (0.5 ug) of TNF- alpha antibody (or ) for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.

Mouse Mono [6N1E7], 100 ug
Hu
FACS, WB

Tumor Necrosis Factor antibody, NBP2-27223, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-27351 Tumor Necrosis Factor antibody, NBP2-27351, Novus Biologicals. Flow Cytometry: TNF-alpha Antibody (6N2E12) [NBP2-27351] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27351. Human PBMC were stimulated with PMA and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with AF647 conjugate.

Tumor Necrosis Factor antibody, NBP2-27351, Novus Biologicals. Flow Cytometry: TNF-alpha Antibody (6N2E12) [NBP2-27351] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27351. Human PBMC were stimulated with PMA and Ionomycin (500 ng/ml) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained with before testing with AF647 conjugate.

Mouse Mono [6N2E12], 100 ug
Hu
FACS, WB

Tumor Necrosis Factor antibody, NBP2-27351, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-34419AF488 Tumor Necrosis Factor antibody, NBP2-34419AF488, Novus Biologicals. Flow Cytometry: TNF-alpha Antibody (SPM543) [Alexa Fluor

Tumor Necrosis Factor antibody, NBP2-34419AF488, Novus Biologicals. Flow Cytometry: TNF-alpha Antibody (SPM543) [Alexa Fluor

Mouse Mono [SPM543], 100 Test
Cn, Fe, Hu, Mo, Rb, Rt, Zf
ELISA, FACS, ICC, IF, IHC-F, IHC-P, IP

ELISA, ICC, IF, IHC-F, IHC-P, IP

Supplier data
Lifespan Biosciences LS-C791407 Tumor Necrosis Factor antibody, LS-C791407, Lifespan Biosciences. HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TNF antibody, and then analyzed by flow cytometry. (1:100)

Tumor Necrosis Factor antibody, LS-C791407, Lifespan Biosciences. HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TNF antibody, and then analyzed by flow cytometry. (1:100)

Mouse Mono [LS-C791407], 1 Vial
Hu
FACS, IHC-F, IHC-P, WB

IHC-F,

Tumor Necrosis Factor antibody, LS-C791407, Lifespan Biosciences, Immunohistochemistry paraffin image

IHC-P,

Tumor Necrosis Factor antibody, LS-C791407, Lifespan Biosciences, Western Blot image

Supplier data
Lifespan Biosciences LS-C794168 Tumor Necrosis Factor antibody, LS-C794168, Lifespan Biosciences. HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TNF antibody, and then analyzed by flow cytometry. (1:100)

Tumor Necrosis Factor antibody, LS-C794168, Lifespan Biosciences. HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TNF antibody, and then analyzed by flow cytometry. (1:100)

Mouse Mono [LS-C794168], 1 Vial
Hu
FACS, IHC-F, IHC-P, WB

IHC-F,

Tumor Necrosis Factor antibody, LS-C794168, Lifespan Biosciences, Immunohistochemistry paraffin image

IHC-P,

Tumor Necrosis Factor antibody, LS-C794168, Lifespan Biosciences, Western Blot image

Supplier data
Lifespan Biosciences LS-C162051 Tumor Necrosis Factor antibody, LS-C162051, Lifespan Biosciences. TNF Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Tumor Necrosis Factor antibody, LS-C162051, Lifespan Biosciences. TNF Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Rabbit Poly, 1 Vial
Hu
FACS, WB

Tumor Necrosis Factor antibody, LS-C162051, Lifespan Biosciences, Western Blot image

Supplier data
Lifespan Biosciences LS-C794170 Tumor Necrosis Factor antibody, LS-C794170, Lifespan Biosciences. HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(blue) were immunostained by anti-TNFA antibody, and then analyzed by flow cytometry. (1:100)

Tumor Necrosis Factor antibody, LS-C794170, Lifespan Biosciences. HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(blue) were immunostained by anti-TNFA antibody, and then analyzed by flow cytometry. (1:100)

Mouse Mono [LS-C794170], 1 Vial
Hu
FACS, WB

Tumor Necrosis Factor antibody, LS-C794170, Lifespan Biosciences, Western Blot image

Supplier data
Lifespan Biosciences LS-C798450 Tumor Necrosis Factor antibody, LS-C798450, Lifespan Biosciences. HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(blue) were immunostained by anti-TNFA antibody, and then analyzed by flow cytometry. (1:100)

Tumor Necrosis Factor antibody, LS-C798450, Lifespan Biosciences. HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(blue) were immunostained by anti-TNFA antibody, and then analyzed by flow cytometry. (1:100)

Mouse Mono [LS-C798450], 1 Vial
Hu
FACS, WB

Tumor Necrosis Factor antibody, LS-C798450, Lifespan Biosciences, Western Blot image

Supplier data
R&D Systems IC210F Tumor Necrosis Factor antibody, IC210F, R&D Systems. Detection of TNF-alpha in Human PBMCs by Flow Cytometry.

Mouse Mono [6402], 100 Test
Hu
FACS

Supplier data
R&D Systems IC410P Tumor Necrosis Factor antibody, IC410P, R&D Systems. Detection of TNF-alpha in EL-4 Mouse Cell Line by Flow Cytometry.

Tumor Necrosis Factor antibody, IC410P, R&D Systems. Detection of TNF-alpha in EL-4 Mouse Cell Line by Flow Cytometry.

Rat Mono [MP6-XT22], 100 Test
Mo
FACS

Supplier data
R&D Systems IC210P Tumor Necrosis Factor antibody, IC210P, R&D Systems. Detection of TNF-alpha in Human PBMCs by Flow Cytometry.

Mouse Mono [6402], 100 Test
Hu
FACS

Supplier data
Lifespan Biosciences LS-C134801 Tumor Necrosis Factor antibody, LS-C134801, Lifespan Biosciences. Flow cytometry of TNF Alpha antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Tumor Necrosis Factor antibody, LS-C134801, Lifespan Biosciences. Flow cytometry of TNF Alpha antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Mono [J1D9], 1 Vial
Hu
FA, FACS, IHC-F, IHC-P, WB

FA, IHC-F, IHC-P, WB

Supplier data
Lifespan Biosciences LS-C134799 Tumor Necrosis Factor antibody, LS-C134799, Lifespan Biosciences. Flow cytometry of TNF Alpha antibody

Tumor Necrosis Factor antibody, LS-C134799, Lifespan Biosciences. Flow cytometry of TNF Alpha antibody

Mouse Mono [J1D9], 1 Vial
Hu
FA, FACS, IHC-F, IHC-P, WB

FA, IHC-F, IHC-P, WB

Supplier data
Lifespan Biosciences LS-C204485 Tumor Necrosis Factor antibody, LS-C204485, Lifespan Biosciences. Intracellular staining of activated human peripheral blood with anti-TNF alpha (MAb11) PE.

Tumor Necrosis Factor antibody, LS-C204485, Lifespan Biosciences. Intracellular staining of activated human peripheral blood with anti-TNF alpha (MAb11) PE.

Mouse Mono [MAb11], 1 Vial
GP, Hu, Pr
FACS

Supplier data
R&D Systems IC410F Tumor Necrosis Factor antibody, IC410F, R&D Systems. Detection of TNF-alpha in EL-4 Mouse Cell Line by Flow Cytometry.

Tumor Necrosis Factor antibody, IC410F, R&D Systems. Detection of TNF-alpha in EL-4 Mouse Cell Line by Flow Cytometry.

Rat Mono [MP6-XT22], 100 Test
Mo
FACS

Supplier data
BioLegend 506348 Tumor Necrosis Factor antibody, 506348, BioLegend. PMA/Ionomycin-stimulatedBALB/c T cells were stained withMP6-XT22 PE

Tumor Necrosis Factor antibody, 506348, BioLegend. PMA/Ionomycin-stimulatedBALB/c T cells were stained withMP6-XT22 PE

Rat Mono [MP6-XT22], 25 ug
Mo
ELISA, FACS, IF, IHC-F, IHC-P, Neu, WB

Tumor Necrosis Factor antibody, 506348, BioLegend, Enzyme Linked Immunosorbent Assay image

ELISA,

Tumor Necrosis Factor antibody, 506348, BioLegend, Immunofluorescence image

IF,

Tumor Necrosis Factor antibody, 506348, BioLegend, Immunohistochemistry frozen image

IHC-F,

Tumor Necrosis Factor antibody, 506348, BioLegend, Immunohistochemistry paraffin image

IHC-P,

Tumor Necrosis Factor antibody, 506348, BioLegend, Neutralising image

Neu,

Tumor Necrosis Factor antibody, 506348, BioLegend, Western Blot image

Supplier data
BioLegend 506347 Tumor Necrosis Factor antibody, 506347, BioLegend. PMA/Ionomycin-stimulatedBALB/c T cells were stained withMP6-XT22 PE

Tumor Necrosis Factor antibody, 506347, BioLegend. PMA/Ionomycin-stimulatedBALB/c T cells were stained withMP6-XT22 PE

Rat Mono [MP6-XT22], 5 ug
Mo
ELISA, FACS, IF, IHC-F, IHC-P, Neu, WB

Tumor Necrosis Factor antibody, 506347, BioLegend, Enzyme Linked Immunosorbent Assay image

ELISA,

Tumor Necrosis Factor antibody, 506347, BioLegend, Immunofluorescence image

IF,

Tumor Necrosis Factor antibody, 506347, BioLegend, Immunohistochemistry frozen image

IHC-F,

Tumor Necrosis Factor antibody, 506347, BioLegend, Immunohistochemistry paraffin image

IHC-P,

Tumor Necrosis Factor antibody, 506347, BioLegend, Neutralising image

Neu,

Tumor Necrosis Factor antibody, 506347, BioLegend, Western Blot image

Supplier data
BioLegend 506349 Tumor Necrosis Factor antibody, 506349, BioLegend. PMA+ Ionomycin-stimulated (six hours) C57BL/6 mouse splenocytes were surface stained with CD3 APC and then intracellularly stained with TNF-α (clone MP6-XT22) Brilliant Violet 711™ (top) or rat IgG1, κ Brilliant Violet 711™ (bottom).

Tumor Necrosis Factor antibody, 506349, BioLegend. PMA+ Ionomycin-stimulated (six hours) C57BL/6 mouse splenocytes were surface stained with CD3 APC and then intracellularly stained with TNF-α (clone MP6-XT22) Brilliant Violet 711™ (top) or rat IgG1, κ Brilliant Violet 711™ (bottom).

Rat Mono [MP6-XT22], 50 ug
Mo
FACS

Supplier data
BioLegend 506108 Tumor Necrosis Factor antibody, 506108, BioLegend. C57 splenocytes were stimulated with Cell Activation Cocktail (with Brefeldin A, Cat# 423303 ) for 6 hours, fixed, permeabilized (Fixation Buffer Cat# 420801, Permeabilization Wash Buffer Cat# 421002), and then stained with TNF-α (clone TN3-19.12) APC (top) or Armenian Hamster IgG APC (bottom).

Tumor Necrosis Factor antibody, 506108, BioLegend. C57 splenocytes were stimulated with Cell Activation Cocktail (with Brefeldin A, Cat# 423303 ) for 6 hours, fixed, permeabilized (Fixation Buffer Cat# 420801, Permeabilization Wash Buffer Cat# 421002), and then stained with TNF-α (clone TN3-19.12) APC (top) or Armenian Hamster IgG APC (bottom).

Hamster Mono [TN3-19.12], 100 ug
Mo, Rb, Rt
FACS

Supplier data
BioLegend 506107 Tumor Necrosis Factor antibody, 506107, BioLegend. C57 splenocytes were stimulated with Cell Activation Cocktail (with Brefeldin A, Cat# 423303 ) for 6 hours, fixed, permeabilized (Fixation Buffer Cat# 420801, Permeabilization Wash Buffer Cat# 421002), and then stained with TNF-α (clone TN3-19.12) APC (top) or Armenian Hamster IgG APC (bottom).

Tumor Necrosis Factor antibody, 506107, BioLegend. C57 splenocytes were stimulated with Cell Activation Cocktail (with Brefeldin A, Cat# 423303 ) for 6 hours, fixed, permeabilized (Fixation Buffer Cat# 420801, Permeabilization Wash Buffer Cat# 421002), and then stained with TNF-α (clone TN3-19.12) APC (top) or Armenian Hamster IgG APC (bottom).

Hamster Mono [TN3-19.12], 25 ug
Mo, Rb, Rt
FACS

Supplier data
Invitrogen Antibodies 56-7349-41

Tumor Necrosis Factor antibody, 56-7349-41, Invitrogen Antibodies. Normal human peripheral blood cells were stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) or unstimulated (left), followed by intracellular staining with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha Alexa Fluor® 700 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [MAb11], 25 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 45-7349-42 Tumor Necrosis Factor antibody, 45-7349-42, Invitrogen Antibodies. Intracellular staining of unstimulated (left) or 5-hour PMA/Ionomycin-stimulated (in the presence of Brefeldin A) (right) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human TNF alpha PerCP-Cyanine5-5. Cells in the monocyte gate were used for analysis.

Tumor Necrosis Factor antibody, 45-7349-42, Invitrogen Antibodies. Intracellular staining of unstimulated (left) or 5-hour PMA/Ionomycin-stimulated (in the presence of Brefeldin A) (right) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human TNF alpha PerCP-Cyanine5-5. Cells in the monocyte gate were used for analysis.

Mouse Mono [MAb11], 100 Test
Hu
FACS

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BioLegend 506333 Tumor Necrosis Factor antibody, 506333, BioLegend. PMA+ Ionomycin-stimulated (6 hours) C57BL/6 mouse splenocytes (in the presence of monensin) were surface stained with CD3 FITC and then intracellularly stained with TNF-α (clone MP6-XT22) Brilliant Violet 650™.

Tumor Necrosis Factor antibody, 506333, BioLegend. PMA+ Ionomycin-stimulated (6 hours) C57BL/6 mouse splenocytes (in the presence of monensin) were surface stained with CD3 FITC and then intracellularly stained with TNF-α (clone MP6-XT22) Brilliant Violet 650™.

Rat Mono [MP6-XT22], 50 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 56-7349-42

Tumor Necrosis Factor antibody, 56-7349-42, Invitrogen Antibodies. Normal human peripheral blood cells were stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) or unstimulated (left), followed by intracellular staining with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha Alexa Fluor® 700 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [MAb11], 100 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 53-7321-82 Tumor Necrosis Factor antibody, 53-7321-82, Invitrogen Antibodies. Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse TNF alpha Alexa Fluor® 488 (filled histogram). Total cells were used for analysis.

Tumor Necrosis Factor antibody, 53-7321-82, Invitrogen Antibodies. Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse TNF alpha Alexa Fluor® 488 (filled histogram). Total cells were used for analysis.

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 25-7349-82 Tumor Necrosis Factor antibody, 25-7349-82, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Tumor Necrosis Factor antibody, 25-7349-82, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Mouse Mono [MAb11], 100 ug
Hu
FACS

Supplier data
NSJ Bioreagents V2903SAF-100UG Tumor Necrosis Factor antibody, V2903SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNFa antibody (TNF706).

Tumor Necrosis Factor antibody, V2903SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNFa antibody (TNF706).

Mouse Mono [TNF706], 100 ug
Bac, Cn, Fe, Hu, Mo, Rb, Rt, Zf
FACS, IF, IHC-P

IF, IHC-P

Supplier data
Invitrogen Antibodies 48-7321-82 Tumor Necrosis Factor antibody, 48-7321-82, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha eFluor® 450 (right).

Tumor Necrosis Factor antibody, 48-7321-82, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha eFluor® 450 (right).

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 25-7423-82

Tumor Necrosis Factor antibody, 25-7423-82, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right), then intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 1 µg of Anti-Mouse/Rat TNF alpha PE-Cyanine7. Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Hamster Mono [TN3-19.12], 100 ug
Mo, Rt
ELISA, FA, FACS, Neu

ELISA, FA, Neu

Supplier data
Invitrogen Antibodies 46-7321-80 Tumor Necrosis Factor antibody, 46-7321-80, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.03 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.03 µg of Anti-Mouse TNF alpha PerCP-eFluor® 710 (right).

Tumor Necrosis Factor antibody, 46-7321-80, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.03 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.03 µg of Anti-Mouse TNF alpha PerCP-eFluor® 710 (right).

Rat Mono [MP6-XT22], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 12-7349-82 Tumor Necrosis Factor antibody, 12-7349-82, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Tumor Necrosis Factor antibody, 12-7349-82, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Mouse Mono [MAb11], 100 ug
Hu
FACS

Supplier data
Invitrogen Antibodies 12-7321-81 Tumor Necrosis Factor antibody, 12-7321-81, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor

Tumor Necrosis Factor antibody, 12-7321-81, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor

Rat Mono [MP6-XT22], 50 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 46-7321-82 Tumor Necrosis Factor antibody, 46-7321-82, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.03 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.03 µg of Anti-Mouse TNF alpha PerCP-eFluor® 710 (right).

Tumor Necrosis Factor antibody, 46-7321-82, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.03 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.03 µg of Anti-Mouse TNF alpha PerCP-eFluor® 710 (right).

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 12-7321-82 Tumor Necrosis Factor antibody, 12-7321-82, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor

Tumor Necrosis Factor antibody, 12-7321-82, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 17-7349-82 Tumor Necrosis Factor antibody, 17-7349-82, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Tumor Necrosis Factor antibody, 17-7349-82, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Mouse Mono [MAb11], 100 ug
Hu
FACS

Supplier data
Invitrogen Antibodies 53-7321-80 Tumor Necrosis Factor antibody, 53-7321-80, Invitrogen Antibodies. Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse TNF alpha Alexa Fluor® 488 (filled histogram). Total cells were used for analysis.

Tumor Necrosis Factor antibody, 53-7321-80, Invitrogen Antibodies. Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse TNF alpha Alexa Fluor® 488 (filled histogram). Total cells were used for analysis.

Rat Mono [MP6-XT22], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 11-7349-81 Tumor Necrosis Factor antibody, 11-7349-81, Invitrogen Antibodies. Normal human blood peripheral cells were stimulated with PMA/Ionomycin in the presence of Brefeldin A (Product # 00-4506-51) for 6 hours. Unstimulated (left) or stimulated (right) cells were surface stained with Anti-Human CD3 PE (Product # 12-0038-42) and intracellularly stained with Anti-Human TNF alpha FITC.

Tumor Necrosis Factor antibody, 11-7349-81, Invitrogen Antibodies. Normal human blood peripheral cells were stimulated with PMA/Ionomycin in the presence of Brefeldin A (Product # 00-4506-51) for 6 hours. Unstimulated (left) or stimulated (right) cells were surface stained with Anti-Human CD3 PE (Product # 12-0038-42) and intracellularly stained with Anti-Human TNF alpha FITC.

Mouse Mono [MAb11], 50 ug
Hu
FACS

Supplier data
Invitrogen Antibodies 12-7423-41

Tumor Necrosis Factor antibody, 12-7423-41, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right), then intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.25 µg of Anti-Mouse/Rat TNF alpha PE. Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Hamster Mono [TN3-19.12], 25 Test
Mo, Rt
FACS

Supplier data
Invitrogen Antibodies 48-7321-80 Tumor Necrosis Factor antibody, 48-7321-80, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha eFluor® 450 (right).

Tumor Necrosis Factor antibody, 48-7321-80, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha eFluor® 450 (right).

Rat Mono [MP6-XT22], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 61-7349-42

Tumor Necrosis Factor antibody, 61-7349-42, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor® 710 (Product # 46-0047-42) and Anti-Human TNF alpha PE-eFluor® 610 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [MAb11], 100 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 53-7349-41

Tumor Necrosis Factor antibody, 53-7349-41, Invitrogen Antibodies. Intracellular staining of 5-hour PMA/Ionomycin stimulated (in the presence of brefeldin A) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) and Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human TNF alpha Alexa Fluor® 488 (right). Total cells were used for analysis.

Mouse Mono [MAb11], 25 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 25-7321-80

Tumor Necrosis Factor antibody, 25-7321-80, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.06 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) or 0.06 µg of Anti-Mouse TNF alpha PE-Cyanine7. Viable cells as determined Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) i

Rat Mono [MP6-XT22], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 45-7349-41 Tumor Necrosis Factor antibody, 45-7349-41, Invitrogen Antibodies. Intracellular staining of unstimulated (left) or 5-hour PMA/Ionomycin-stimulated (in the presence of Brefeldin A) (right) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human TNF alpha PerCP-Cyanine5-5. Cells in the monocyte gate were used for analysis.

Tumor Necrosis Factor antibody, 45-7349-41, Invitrogen Antibodies. Intracellular staining of unstimulated (left) or 5-hour PMA/Ionomycin-stimulated (in the presence of Brefeldin A) (right) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human TNF alpha PerCP-Cyanine5-5. Cells in the monocyte gate were used for analysis.

Mouse Mono [MAb11], 25 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 50-7423-82

Tumor Necrosis Factor antibody, 50-7423-82, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right), then intracellularly stained with Anti-Mouse CD4 PE (Product # 48-0041-82) and 0.25 µg of Anti-Mouse/Rat TNF alpha eFluor® 660. Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Hamster Mono [TN3-19.12], 100 ug
Mo, Rt
FACS

Supplier data
Invitrogen Antibodies 61-7321-82

Tumor Necrosis Factor antibody, 61-7321-82, Invitrogen Antibodies. Mouse splenocytes were stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03). Cells were intracellularly stained with Anti-Mouse CD4 APC (Product # 17-0042-82) and 0.125 µg of Rat IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha PE-eFluor® 610 (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte ga

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 25-7321-82

Tumor Necrosis Factor antibody, 25-7321-82, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.06 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) or 0.06 µg of Anti-Mouse TNF alpha PE-Cyanine7. Viable cells as determined Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) i

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 12-7349-81 Tumor Necrosis Factor antibody, 12-7349-81, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Tumor Necrosis Factor antibody, 12-7349-81, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor

Mouse Mono [MAb11], 50 ug
Hu
FACS

Supplier data
Invitrogen Antibodies 11-7349-82 Tumor Necrosis Factor antibody, 11-7349-82, Invitrogen Antibodies. Normal human blood peripheral cells were stimulated with PMA/Ionomycin in the presence of Brefeldin A (Product # 00-4506-51) for 6 hours. Unstimulated (left) or stimulated (right) cells were surface stained with Anti-Human CD3 PE (Product # 12-0038-42) and intracellularly stained with Anti-Human TNF alpha FITC.

Tumor Necrosis Factor antibody, 11-7349-82, Invitrogen Antibodies. Normal human blood peripheral cells were stimulated with PMA/Ionomycin in the presence of Brefeldin A (Product # 00-4506-51) for 6 hours. Unstimulated (left) or stimulated (right) cells were surface stained with Anti-Human CD3 PE (Product # 12-0038-42) and intracellularly stained with Anti-Human TNF alpha FITC.

Mouse Mono [MAb11], 100 ug
Hu
FACS

Supplier data
Invitrogen Antibodies 11-7423-82

Tumor Necrosis Factor antibody, 11-7423-82, Invitrogen Antibodies. Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right), then intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.125 µg of Anti-Mouse/Rat TNF alpha FITC. Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

Hamster Mono [TN3-19.12], 100 ug
Mo, Rt
FACS

Supplier data
Invitrogen Antibodies 11-7321-81

Tumor Necrosis Factor antibody, 11-7321-81, Invitrogen Antibodies. Mouse splenocytes were stimulated with ConA for 2 days, followed by Mouse IL-2 Recombinant Protein (Product # 14-8021-64) and Mouse IL-4 Recombinant Protein (Product # 14-8041-80) for 3 days, and re-stimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0032-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A (Product # 00-4506-51) for 5 hours. Cells were stained with Anti-Mouse CD4 PE (Product # 12-0041-82)

Rat Mono [MP6-XT22], 50 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 14-7321-81

Tumor Necrosis Factor antibody, 14-7321-81, Invitrogen Antibodies. Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Mouse CD4 FITC (Product # 11-0041-82) and intracellularly stained with Anti-Mouse TNF alpha PE. (right). The cells were surface stained with Anti-

Rat Mono [MP6-XT22], 50 ug
Mo
FACS, IHC-F

IHC-F

Supplier data
Invitrogen Antibodies 17-7321-81 Tumor Necrosis Factor antibody, 17-7321-81, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control APC (Product # 17-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha APC (right).

Tumor Necrosis Factor antibody, 17-7321-81, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control APC (Product # 17-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha APC (right).

Rat Mono [MP6-XT22], 50 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 53-7349-42

Tumor Necrosis Factor antibody, 53-7349-42, Invitrogen Antibodies. Intracellular staining of 5-hour PMA/Ionomycin stimulated (in the presence of brefeldin A) normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) and Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human TNF alpha Alexa Fluor® 488 (right). Total cells were used for analysis.

Mouse Mono [MAb11], 100 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 14-7321-85

Tumor Necrosis Factor antibody, 14-7321-85, Invitrogen Antibodies. Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Mouse CD4 FITC (Product # 11-0041-82) and intracellularly stained with Anti-Mouse TNF alpha PE. (right). The cells were surface stained with Anti-

Rat Mono [MP6-XT22], 500 ug
Mo
FACS, IHC-F

IHC-F

Supplier data
Invitrogen Antibodies 48-7349-41 Tumor Necrosis Factor antibody, 48-7349-41, Invitrogen Antibodies. Intracellular staining of human peripheral blood cells unstimulated (left) or stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha eFluor® 450. Cells in the lymphocyte gate were used for analysis.

Tumor Necrosis Factor antibody, 48-7349-41, Invitrogen Antibodies. Intracellular staining of human peripheral blood cells unstimulated (left) or stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha eFluor® 450. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [MAb11], 25 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 11-7321-82

Tumor Necrosis Factor antibody, 11-7321-82, Invitrogen Antibodies. Mouse splenocytes were stimulated with ConA for 2 days, followed by Mouse IL-2 Recombinant Protein (Product # 14-8021-64) and Mouse IL-4 Recombinant Protein (Product # 14-8041-80) for 3 days, and re-stimulated with immobilized Anti-Mouse CD3 Functional Grade Purified (Product # 16-0032-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A (Product # 00-4506-51) for 5 hours. Cells were stained with Anti-Mouse CD4 PE (Product # 12-0041-82)

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 17-7321-82 Tumor Necrosis Factor antibody, 17-7321-82, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control APC (Product # 17-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha APC (right).

Tumor Necrosis Factor antibody, 17-7321-82, Invitrogen Antibodies. Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.125 µg of Rat IgG1 K Isotype Control APC (Product # 17-4301-82) (left) or 0.125 µg of Anti-Mouse TNF alpha APC (right).

Rat Mono [MP6-XT22], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 48-7349-42 Tumor Necrosis Factor antibody, 48-7349-42, Invitrogen Antibodies. Intracellular staining of human peripheral blood cells unstimulated (left) or stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha eFluor® 450. Cells in the lymphocyte gate were used for analysis.

Tumor Necrosis Factor antibody, 48-7349-42, Invitrogen Antibodies. Intracellular staining of human peripheral blood cells unstimulated (left) or stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha eFluor® 450. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [MAb11], 100 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 61-7349-41

Tumor Necrosis Factor antibody, 61-7349-41, Invitrogen Antibodies. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Cells were intracellularly stained with Anti-Human CD4 PerCP-eFluor® 710 (Product # 46-0047-42) and Anti-Human TNF alpha PE-eFluor® 610 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [MAb11], 25 Test
Hu
FACS

Supplier data
NSJ Bioreagents V2898SAF-100UG Tumor Necrosis Factor antibody, V2898SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha antibody (TNFA/1172)

Tumor Necrosis Factor antibody, V2898SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha antibody (TNFA/1172)

Mouse Mono [TNFA/1172], 100 ug
Bac, Hu, Rt
FACS, IF, IHC-P

IF, IHC-P

Supplier data
NSJ Bioreagents V2900SAF-100UG Tumor Necrosis Factor antibody, V2900SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF-a antibody (P/T2).

Tumor Necrosis Factor antibody, V2900SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF-a antibody (P/T2).

Mouse Mono [P/T2], 100 ug
Bac, Cn, Fe, Hu, Mo, Rb, Rt, Zf
FACS, IF, IHC-P

IF, IHC-P

Supplier data
NSJ Bioreagents V2900-100UG Tumor Necrosis Factor antibody, V2900-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF-a antibody (P/T2).

Tumor Necrosis Factor antibody, V2900-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF-a antibody (P/T2).

Mouse Mono [P/T2], 100 ug
Bac, Cn, Fe, Hu, Mo, Rb, Rt, Zf
FACS, IF, IHC-P

IF, IHC-P

Supplier data
NSJ Bioreagents V2898-100UG Tumor Necrosis Factor antibody, V2898-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha antibody (TNFA/1172)

Tumor Necrosis Factor antibody, V2898-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha antibody (TNFA/1172)

Mouse Mono [TNFA/1172], 100 ug
Bac, Hu, Rt
FACS, IF, IHC-P

IF, IHC-P

Show/hide more from NSJ Bioreagents

Supplier data
NSJ Bioreagents V2903-100UG Tumor Necrosis Factor antibody, V2903-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNFa antibody (TNF706).

Tumor Necrosis Factor antibody, V2903-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNFa antibody (TNF706).

Mouse Mono [TNF706], 100 ug
Bac, Cn, Fe, Hu, Mo, Rb, Rt, Zf
FACS, IF, IHC-P

IF, IHC-P

Supplier data
NSJ Bioreagents V2904-100UG Tumor Necrosis Factor antibody, V2904-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha antibody (TNF706 + P/T2).

Tumor Necrosis Factor antibody, V2904-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha antibody (TNF706 + P/T2).

Mouse Mono [TNF706 + P/T2], 100 ug
Bac, Cn, Fe, Hu, Mo, Rb, Rt, Zf
FACS, IF, IHC-P

IF, IHC-P

Supplier data
NSJ Bioreagents V2904SAF-100UG Tumor Necrosis Factor antibody, V2904SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha antibody (TNF706 + P/T2).

Tumor Necrosis Factor antibody, V2904SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha antibody (TNF706 + P/T2).

Mouse Mono [TNF706 + P/T2], 100 ug
Bac, Cn, Fe, Hu, Mo, Rb, Rt, Zf
FACS, IF, IHC-P

IF, IHC-P

Supplier data
Cell Signaling Technology 11948T Tumor Necrosis Factor antibody, 11948T, Cell Signaling Technology.

Rabbit Mono [11948T], 20 ul
Ha
AP, FACS, ICC, IF, IP, WB

AP,

Tumor Necrosis Factor antibody, 11948T, Cell Signaling Technology, Immunocytochemistry image

ICC,

Tumor Necrosis Factor antibody, 11948T, Cell Signaling Technology, Immunofluorescence image

IF,

Tumor Necrosis Factor antibody, 11948T, Cell Signaling Technology, Immunoprecipitation image

IP,

Tumor Necrosis Factor antibody, 11948T, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 11948S Tumor Necrosis Factor antibody, 11948S, Cell Signaling Technology.

Rabbit Mono [D2D4], 100 ul
Mo, Rt
FACS, ICC, IF, IP, WB

Tumor Necrosis Factor antibody, 11948S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Tumor Necrosis Factor antibody, 11948S, Cell Signaling Technology, Immunofluorescence image

IF,

Tumor Necrosis Factor antibody, 11948S, Cell Signaling Technology, Immunoprecipitation image

IP,

Tumor Necrosis Factor antibody, 11948S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 8184S Tumor Necrosis Factor antibody, 8184S, Cell Signaling Technology.

Rabbit Mono [D1G2], 100 ul
Hu
FACS, ICC, IF, IP, WB

Tumor Necrosis Factor antibody, 8184S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Tumor Necrosis Factor antibody, 8184S, Cell Signaling Technology, Immunofluorescence image

IF, IP,

Tumor Necrosis Factor antibody, 8184S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 11948P Tumor Necrosis Factor antibody, 11948P, Cell Signaling Technology.

Rabbit Mono [D2D4], 40 ul
Mo, Rt
AP, FACS, IF, IP, WB

AP,

Tumor Necrosis Factor antibody, 11948P, Cell Signaling Technology, Immunofluorescence image

IF,

Tumor Necrosis Factor antibody, 11948P, Cell Signaling Technology, Immunoprecipitation image

IP,

Tumor Necrosis Factor antibody, 11948P, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 12685S Tumor Necrosis Factor antibody, 12685S, Cell Signaling Technology.

Rabbit Mono [D1G2], 100 ul
Hu
FACS, IF

Tumor Necrosis Factor antibody, 12685S, Cell Signaling Technology, Immunofluorescence image

Supplier data
Cell Signaling Technology 12398S Tumor Necrosis Factor antibody, 12398S, Cell Signaling Technology.

Rabbit Mono [D1G2], 100 ul
Hu
FACS

Supplier data
Cell Signaling Technology 12779S Tumor Necrosis Factor antibody, 12779S, Cell Signaling Technology.

Rabbit Mono [D2D4], 100 ul
Mo, Rt
FACS

Related antibodies

Tumor Necrosis Factor antibodies are often published with	Associated citations
IL-6	9.5%
IL-12	4.8%
Calcitonin	3.8%
CD20	3.4%
NADH:Ubiquinone Oxidoreductase Subunit A11	3.0%
Mitogen-Activated Protein Kinase 8 Interacting Protein 3	2.9%
2 -5 -oligoadenylate synthase 1A	2.9%
Vitamin K Epoxide Reductase Complex Subunit 1 Like 1	2.9%
LON Peptidase N-Terminal Domain And Ring Finger 1	2.9%
Ubiquitin Conjugating Enzyme E2 L6	2.8%

Featured Research

anti-Tumor Necrosis Factor Antibody
sc-133192 (Santa Cruz Biotechnology)
Rt / EMSA
doi:10.1016/j.ejpb.2013.05.003

anti-Tumor Necrosis Factor Antibody
430901 (BioLegend)
Mo / ELISA
doi:10.1007/s10753-013-9732-x

anti-Tumor Necrosis Factor Antibody
RTA00 (R&D Systems)
Hu / ELISA
doi:10.1007/s10620-013-2871-y

anti-Tumor Necrosis Factor Antibody
430901 (BioLegend)
Mo / ELISA
doi:10.1016/j.intimp.2013.04.020

anti-Tumor Necrosis Factor Antibody
ab6671 (Abcam)
Hu / IF
doi:10.2337/db12-0844

anti-Tumor Necrosis Factor Antibody
88-7324-77 (eBioscience)
Mo / ELISA
doi:10.1111/bph.12110

anti-Tumor Necrosis Factor Antibody
506307 (BioLegend)
Mo / FACS
doi:10.1371/journal.pone.0063269

anti-Tumor Necrosis Factor Antibody
506104 (BioLegend)
Rt / FACS
doi:10.1111/sji.12123

anti-Tumor Necrosis Factor Antibody
506313 (BioLegend)
Mo / FACS
doi:10.4049/jimmunol.1201153

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

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Antibody Resource Tumor Necrosis Factor Antibody Comparison using Flow Cytometry

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	WB	FACS	ELISA	IHC-P	IHC-F	IF	ICC	IP	AP	Neu	FA	Bl	RIA	IVT	Pep-ELISA	ChIP	DB	EMSA
Human	686	674	673	571	477	464	284	117	62	50	51	15	9	9	7	7	4	1
Mouse	361	299	300	282	229	250	190	85	49	62	28		5	9	5	5	3	1
Rat	240	176	195	284	206	270	190	57	27	29	17		1		5	4	1	1
Rabbit	34	93	53	94	74	87	55	45	17	8
Primate	61	93	54	45	50	35	57	23	13	3	6		2			5	1	1
Canine	36	75	60	90	73	82	53	40	18	3	2					5	1	1
Zebrafish	2	69	31	71	55	69	50	27	4							4	1	1
Feline	10	61	31	69	56	61	42	39	17
Bacteria	10	67	9	22	10	29		4		8
Bovine	38	24	53	12	11	9	7	2	2	1	2					1
Guinea Pig	44	23	41	23	27	11	15	4	1	3	3					4	1	1
Porcine	26	25	34	11	12	26	39	2	4	3	4
Sheep	25	7	25	3	7	1	1	1	2
Equine	21	1	14	4	2	2	2	1	1	1
Hamster	5	3	18	18	18	20	19	3	2		1
Chicken	10	2	6	6	6	3	5	2		1						4	1	1
Fish	8		1	10	10	9	9
Donkey	5		3	3	3			2	1
Goat	3							1	1
Mammals	1							1	1
Eukaryotes	1							1	1
Squirrel	1							1	1
Camel	1							1	1
Llama	1							1	1
Fungus	1							1	1
Vertebrates	1							1	1