Interleukin 21 Antibodies for Flow Cytometry

71 matching Interleukin 21 antibodies for Flow Cytometry after applying filters

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Supplier data
R&D Systems MAB594 Interleukin 21 antibody, MAB594, R&D Systems. Detection of IL-21 in Activated Mouse Splenocytes by Flow Cytometry.

Rat Mono [149204], 500 ug
Mo
FACS, WB

Interleukin 21 antibody, MAB594, R&D Systems, Western Blot image

Supplier data
Invitrogen Antibodies 50-7211-80

Interleukin 21 antibody, 50-7211-80, Invitrogen Antibodies. Intracellular staining of 10-day Th17-polarized BALB/c splenocytes unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) or 0.125 µg of Anti-Mouse IL-21 eFluor® 660. Viable cells (as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14) were

Rat Mono [FFA21], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 17-7211-80

Interleukin 21 antibody, 17-7211-80, Invitrogen Antibodies. Intracellular staining of Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.125 µg of Anti-Mouse IL-21 APC. Cells were polarized for 9 days and then treated for 5 hours with Brefeldin A alone (Product # 00-4506-51) (left) or PMA/Ionomycin and Brefeldin A (right). Cells in the lymphocyte gate were used for analysis.

Rat Mono [FFA21], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 50-7213-82

Interleukin 21 antibody, 50-7213-82, Invitrogen Antibodies. Mouse splenocytes were cultured under Th17-polarizing conditions for 7 days, then treated for 5 hours with the Protein Transport Inhibtor Cocktail (Product # 00-4980-03) (left) or the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right). Intracellular staining with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.25 µg of Anti-Mouse IL-21 eFluor® 660 was performed using the Fixation and Permeabilization Buffers (Product # 88-8823-88) and protocol

Rat Mono [mhalx21], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 12-7219-41 Interleukin 21 antibody, 12-7219-41, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells treated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-42) (left) or Anti-Human IL-21 PE (right). Cells in the lymphocyte gate were used for analysis.

Interleukin 21 antibody, 12-7219-41, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells treated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-42) (left) or Anti-Human IL-21 PE (right). Cells in the lymphocyte gate were used for analysis.

Mouse Mono [eBio3A3-N2 (3A3-N2)], 25 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 50-7211-82

Interleukin 21 antibody, 50-7211-82, Invitrogen Antibodies. Intracellular staining of 10-day Th17-polarized BALB/c splenocytes unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) or 0.125 µg of Anti-Mouse IL-21 eFluor® 660. Viable cells (as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14) were

Rat Mono [FFA21], 100 ug
Mo
FACS

Supplier data
Abbexa abx034164 Interleukin 21 antibody, abx034164, Abbexa.

Rabbit Poly, 400 ul
Hu
FACS, WB

Interleukin 21 antibody, abx034164, Abbexa, Western Blot image

Supplier data
Invitrogen Antibodies 12-7211-80

Interleukin 21 antibody, 12-7211-80, Invitrogen Antibodies. Intracellular staining of 11-day Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse IL-21 PE. Cultures were treated for 5 hours prior to harvest with either Brefeldin A (Product # 00-4506-51) alone (left) or PMA, Ionomycin, and Brefeldin A (right). Cells in the lymphocyte gate were used for analysis.

Rat Mono [FFA21], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 12-7213-82

Interleukin 21 antibody, 12-7213-82, Invitrogen Antibodies. Staining of 12-day Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse IL-21 PE. Cells were treated with Brefeldin A (Product # 00-4506-51) alone (left) or PMA, Ionomycin, and Brefeldin A (right) for 5 hours prior to intracellular staining. Cells in the lymphocyte gate were used for analysis.

Rat Mono [mhalx21], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 50-7219-42 Interleukin 21 antibody, 50-7219-42, Invitrogen Antibodies. Intracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

Interleukin 21 antibody, 50-7219-42, Invitrogen Antibodies. Intracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [eBio3A3-N2 (3A3-N2)], 100 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 12-7211-82

Interleukin 21 antibody, 12-7211-82, Invitrogen Antibodies. Intracellular staining of 11-day Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse IL-21 PE. Cultures were treated for 5 hours prior to harvest with either Brefeldin A (Product # 00-4506-51) alone (left) or PMA, Ionomycin, and Brefeldin A (right). Cells in the lymphocyte gate were used for analysis.

Rat Mono [FFA21], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 17-7211-82

Interleukin 21 antibody, 17-7211-82, Invitrogen Antibodies. Intracellular staining of Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.125 µg of Anti-Mouse IL-21 APC. Cells were polarized for 9 days and then treated for 5 hours with Brefeldin A alone (Product # 00-4506-51) (left) or PMA/Ionomycin and Brefeldin A (right). Cells in the lymphocyte gate were used for analysis.

Rat Mono [FFA21], 100 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 12-7219-42 Interleukin 21 antibody, 12-7219-42, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells treated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-42) (left) or Anti-Human IL-21 PE (right). Cells in the lymphocyte gate were used for analysis.

Interleukin 21 antibody, 12-7219-42, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells treated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-42) (left) or Anti-Human IL-21 PE (right). Cells in the lymphocyte gate were used for analysis.

Mouse Mono [eBio3A3-N2 (3A3-N2)], 100 Test
Hu
FACS

Supplier data
Invitrogen Antibodies 12-7213-80

Interleukin 21 antibody, 12-7213-80, Invitrogen Antibodies. Staining of 12-day Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse IL-21 PE. Cells were treated with Brefeldin A (Product # 00-4506-51) alone (left) or PMA, Ionomycin, and Brefeldin A (right) for 5 hours prior to intracellular staining. Cells in the lymphocyte gate were used for analysis.

Rat Mono [mhalx21], 25 ug
Mo
FACS

Supplier data
Invitrogen Antibodies 50-7219-41 Interleukin 21 antibody, 50-7219-41, Invitrogen Antibodies. Intracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

Interleukin 21 antibody, 50-7219-41, Invitrogen Antibodies. Intracellular staining of Th17-polarized normal human peripheral blood cells restimulated with monensin alone (left) or PMA/Ionomycin and monensin for 5 hours (right) with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-21 eFluor® 660. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [eBio3A3-N2 (3A3-N2)], 25 Test
Hu
FACS

Western Blot

Supplier data
R&D Systems MAB594

Interleukin 21 antibody, MAB594, R&D Systems. Detection of IL-21 in Activated Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were untreated (light orange filled histogram) or activated for 24 hours with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin (dark orange filled histogram) then stained with Mouse IL-21 Monoclonal Antibody (Catalog # MAB594) or isotype control antibody (Catalog # <a class="NoLineLink" href="http://www.rndsystems.com/search?keywords=MAB0061">MAB0061</a>, open histogram), followed by Allophycocyanin-conjugated Anti-R

Rat Mono [149204], 500 ug
Mo
FACS, WB

Interleukin 21 antibody, MAB594, R&D Systems, Flow Cytometry image

FACS

Supplier data
Abbexa abx034164 Interleukin 21 antibody, abx034164, Abbexa.

Rabbit Poly, 400 ul
Hu
FACS, WB

Interleukin 21 antibody, abx034164, Abbexa, Flow Cytometry image

FACS

Immunocytochemistry

Supplier data
BioLegend 513004 Interleukin 21 antibody, 513004, BioLegend. PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2

Interleukin 21 antibody, 513004, BioLegend. PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2

Mouse Mono [3A3-N2], 100 Test
Hu, Pr
FACS, ICC

FACS

(1)

1 review

Supplier data
BioLegend 513007 Interleukin 21 antibody, 513007, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were intracellularly stained with IL-21(clone 3A3-N2) APC (top) or mouse IgG1 APC isotype control (bottom) and CD3 FITC.

Interleukin 21 antibody, 513007, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were intracellularly stained with IL-21(clone 3A3-N2) APC (top) or mouse IgG1 APC isotype control (bottom) and CD3 FITC.

Mouse Mono [3A3-N2], 25 Test
Hu, Pr
FACS, ICC

FACS

Supplier data
BioLegend 513003 Interleukin 21 antibody, 513003, BioLegend. PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2

Interleukin 21 antibody, 513003, BioLegend. PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2

Mouse Mono [3A3-N2], 25 Test
Hu, Pr
FACS, ICC

FACS

Supplier data
BioLegend 513005 Interleukin 21 antibody, 513005, BioLegend. PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with 3A3-N2 Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom) and CD3 (UCHT1) PE

Interleukin 21 antibody, 513005, BioLegend. PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with 3A3-N2 Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom) and CD3 (UCHT1) PE

Mouse Mono [3A3-N2], 25 Test
Hu, Pr
FACS, ICC

FACS

Supplier data
BioLegend 513008 Interleukin 21 antibody, 513008, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were intracellularly stained with IL-21(clone 3A3-N2) APC (top) or mouse IgG1 APC isotype control (bottom) and CD3 FITC.

Interleukin 21 antibody, 513008, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were intracellularly stained with IL-21(clone 3A3-N2) APC (top) or mouse IgG1 APC isotype control (bottom) and CD3 FITC.

Mouse Mono [3A3-N2], 100 Test
Hu, Pr
FACS, ICC

FACS

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Supplier data
BioLegend 516703 Interleukin 21 antibody, 516703, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 APC, then intracellularly stained with 4BG1 PE

Interleukin 21 antibody, 516703, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 APC, then intracellularly stained with 4BG1 PE

Mouse Mono [4BG1], 25 Test
Hu
FACS, ICC

FACS

Supplier data
BioLegend 513006 Interleukin 21 antibody, 513006, BioLegend. PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with 3A3-N2 Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom) and CD3 (UCHT1) PE

Interleukin 21 antibody, 513006, BioLegend. PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellular stained with 3A3-N2 Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom) and CD3 (UCHT1) PE

Mouse Mono [3A3-N2], 100 Test
Hu, Pr
FACS, ICC

FACS

Supplier data
BioLegend 516704 Interleukin 21 antibody, 516704, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 APC, then intracellularly stained with 4BG1 PE

Interleukin 21 antibody, 516704, BioLegend. PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 APC, then intracellularly stained with 4BG1 PE

Mouse Mono [4BG1], 100 Test
Hu
FACS, ICC

FACS

Interleukin 21 antibodies are often published with	Associated citations
CD3	19.8%
CD19	13.2%
IL-17	9.0%
IL-4	9.0%
His tag	7.8%
CD8	7.8%
CD28	7.2%
IL-12	6.6%
Inducible T Cell Costimulator	6.6%
IL-6	6.0%

Interleukin 21 antibodies are often published with

Associated citations

19.8%

13.2%

9.0%

9.0%

7.8%

7.8%

7.2%

6.6%

Inducible T Cell Costimulator

6.6%

IL-6

6.0%

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

71 matching Interleukin 21 antibodies for Flow Cytometry from 21 suppliers

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1 Applications, 10 Species currently selected

71 matching Interleukin 21 antibodies for Flow Cytometry after applying filters

Flow Cytometry - Use Filter to switch application

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Immunocytochemistry

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	WB	ELISA	IHC-P	ICC	IHC-F	FACS	IF	Neu	FA
Human	47	29	23	22	17	13	10
Mouse	24	19	11	5	7	10	7	4	2
Rat	10	3	10	4	5		5
Primate		4		6		6
Bovine	4	4	1
Canine	1	3	1					1
Equine	1	1
Rabbit	1		1		1		1
Porcine	1	1	1
Guinea Pig	1	1