His tag Antibodies for Immunofluorescence

57 matching His tag antibodies for Immunofluorescence after applying filters

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Immunofluorescence - Use Filter to switch application

Supplier data
BioLegend 362611

His tag antibody, 362611, BioLegend. HeLa cells transiently transfected with His tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 647 anti- His tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 40X objective. For negative control and titration data, refer t

Mouse Mono [J095G46], 25 ug
Mam
IF

Supplier data
BioLegend 652511

His tag antibody, 652511, BioLegend. HeLa cells transiently transfected with His tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:2500 diluted (0.2 µg/ml) Alexa Fluor® 594 anti- His tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 40X objective.

Mouse Mono [J099B12], 25 ug
Hu, Mam, Pr
IF

Supplier data
BioLegend 652509

His tag antibody, 652509, BioLegend. HeLa cells transiently transfected with His tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 488 anti- His tag antibody (green). Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 40X objective.

Mouse Mono [J099B12], 25 ug
Hu, Mam, Pr
IF

Supplier data
BioLegend 362609 His tag antibody, 362609, BioLegend.

Mouse Mono [J095G46], 25 ug
Hu, Mam, Pr
IF

Supplier data
BioLegend 652513

His tag antibody, 652513, BioLegend. HeLa cells transiently transfected with His tag fused protein were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 647 anti- His tag antibody (red). Nuclei were counterstained with DAPI (blue, Cat no. 422801). The image was captured with a 40X objective.

Mouse Mono [J099B12], 25 ug
Hu, Mam, Pr
IF

Supplier data
Proteintech Group 66005-1-Ig His tag antibody, 66005-1-Ig, Proteintech Group.

Mouse Mono [1B7G5], 150 ul
Hu
ELISA, IF, IP, WB

ELISA,

His tag antibody, 66005-1-Ig, Proteintech Group, Immunoprecipitation image

IP,

His tag antibody, 66005-1-Ig, Proteintech Group, Western Blot image

Supplier data
Cell Signaling Technology 12698S His tag antibody, 12698S, Cell Signaling Technology.

Rabbit Mono [D3I1O], 100 ul
Ch
ChIP, FACS, ICC, IF, IP, WB

His tag antibody, 12698S, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

His tag antibody, 12698S, Cell Signaling Technology, Flow Cytometry image

FACS,

His tag antibody, 12698S, Cell Signaling Technology, Immunocytochemistry image

ICC,

His tag antibody, 12698S, Cell Signaling Technology, Immunoprecipitation image

IP,

His tag antibody, 12698S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 14931S His tag antibody, 14931S, Cell Signaling Technology.

Rabbit Mono [14931S], 100 ul
Ch
FACS, ICC, IF

His tag antibody, 14931S, Cell Signaling Technology, Flow Cytometry image

FACS,

His tag antibody, 14931S, Cell Signaling Technology, Immunocytochemistry image

ICC

Supplier data
Cell Signaling Technology 14930S His tag antibody, 14930S, Cell Signaling Technology.

Rabbit Mono [14930S], 100 ul
Ch
FACS, ICC, IF

His tag antibody, 14930S, Cell Signaling Technology, Flow Cytometry image

FACS,

His tag antibody, 14930S, Cell Signaling Technology, Immunocytochemistry image

ICC

Supplier data
GeneTex GTX628914 His tag antibody, GTX628914, GeneTex. 6X His tag antibody [GT359] detects 6X His tag protein by immunofluorescent analysis.</br>Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: 6X His tag stained by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.</br>Blue: Fluoroshield with DAPI (GTX30920).

His tag antibody, GTX628914, GeneTex. 6X His tag antibody [GT359] detects 6X His tag protein by immunofluorescent analysis.</br>Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: 6X His tag stained by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.</br>Blue: Fluoroshield with DAPI (GTX30920).

Mouse Mono [GT359], 100 ul
Hu
ELISA, ICC, IF, IP, WB

His tag antibody, GTX628914, GeneTex, Enzyme Linked Immunosorbent Assay image

ELISA,

His tag antibody, GTX628914, GeneTex, Immunocytochemistry image

ICC,

His tag antibody, GTX628914, GeneTex, Immunoprecipitation image

IP,

Western Blot

Supplier data
Origene AM20706PU-S His tag antibody, AM20706PU-S, Origene.

Mouse Poly, 50 ug
Mam
IF, IP, WB

IF, IP

Supplier data
MyBioSource MBS125007 His tag antibody, MBS125007, MyBioSource. Western blot analysis of over-expressed His-tagged protein in 293T cell using His antibody at different dilution. Each lane was loaded with 2 ug cell lysate.

His tag antibody, MBS125007, MyBioSource. Western blot analysis of over-expressed His-tagged protein in 293T cell using His antibody at different dilution. Each lane was loaded with 2 ug cell lysate.

Mouse Mono [MBS125007], 50 ug
Hu, Mo, Rt
IF, IP, WB

IF, IP

Supplier data
Novus Biologicals NBP2-43536 His tag antibody, NBP2-43536, Novus Biologicals. Western Blot: His Tag Antibody (359) [NBP2-43536] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His Tag antibody (359). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

His tag antibody, NBP2-43536, Novus Biologicals. Western Blot: His Tag Antibody (359) [NBP2-43536] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His Tag antibody (359). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Mouse Mono [359], 100 ul
Hu
ELISA, ICC, IF, WB

His tag antibody, NBP2-43536, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

His tag antibody, NBP2-43536, Novus Biologicals, Immunocytochemistry image

ICC, IF

Supplier data
BioLegend 362601

His tag antibody, 362601, BioLegend. Total cell lysate (15 µg protein) from 293E (lane 1) and 293E transfected with His-tag fused protein (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL), 1:2500 diluted (0.2 µg/mL) and 1:10000 diluted (0.0 5 µg/mL) purified anti-His tag antibody (clone J095G46). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat#405306,

Mouse Mono [J095G46], 25 ug
Mam
IF, WB

Supplier data
BioLegend 362602

His tag antibody, 362602, BioLegend. Total cell lysate (15 µg protein) from 293E (lane 1) and 293E transfected with His-tag fused protein (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL), 1:2500 diluted (0.2 µg/mL) and 1:10000 diluted (0.0 5 µg/mL) purified anti-His tag antibody (clone J095G46). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat#405306,

Mouse Mono [J095G46], 100 ug
Mam
IF, WB

Supplier data
Proteintech Group 66005-1-Ig His tag antibody, 66005-1-Ig, Proteintech Group. The 6*His, His-Tag antibody from Proteintech is a mouse monoclonal antibody to a peptide of n/a 6*His, His-Tag. This antibody recognizes recombinant protein antigen. The 6*His, His-Tag antibody has been validated for the following applications: IF, IP, WB, ELISA analysis.

His tag antibody, 66005-1-Ig, Proteintech Group. The 6*His, His-Tag antibody from Proteintech is a mouse monoclonal antibody to a peptide of n/a 6*His, His-Tag. This antibody recognizes recombinant protein antigen. The 6*His, His-Tag antibody has been validated for the following applications: IF, IP, WB, ELISA analysis.

Mouse Mono [1B7G5], 150 ul
Hu
ELISA, IF, IP, WB

ELISA,

His tag antibody, 66005-1-Ig, Proteintech Group, Immunofluorescence image

IF,

Supplier data
Cell Signaling Technology 12698S His tag antibody, 12698S, Cell Signaling Technology.

Rabbit Mono [D3I1O], 100 ul
Ch
ChIP, FACS, ICC, IF, IP, WB

ChIP,

FACS,

ICC,

His tag antibody, 12698S, Cell Signaling Technology, Immunofluorescence image

IF,

Related antibodies

His tag antibodies are often published with	Associated citations
CD3	44.1%
Secretoglobin Family 1A Member 1	11.5%
CD8	7.8%
CD25	6.8%
IL-4	6.6%
3-Hydroxy-3-Methylglutaryl-CoA Reductase	5.7%
PMP-22	5.6%
IL-17	3.5%
CD19	3.5%
ATP Binding Cassette Subfamily G Member 2 (Junior Blood Group)	3.2%

All None
	WB	ELISA	IF	FACS	ICC	IP	IHC-F	IHC-P	DB	AP	ChIP
Human	68	46	41	34	34	13	8	7
Chicken	43	5	31	37	28	14	1	1	1		1
Mouse	28	10	17	16	12	14	2	2
Bacteria	23	15	8	10	12	4	10	8
Sheep	21	15	19	19	16	5	2	2
Mammals	19	1	13	12	5	8	2			1
Rat	17	3	14	13	12	7	2	2
Primate	10	1	9	7	2	4
Drosophila	10	4	5	10	2	4	4	2
Goat	10	3	6	5	2	4
Virus	9	1	4	5	3	4	1	1
Goldfish	8		4	5	2	4
Equine	8	1	5	5	2	4
Bovine	8	1	5	6	2	4
Frog	8	1	5	5	2	4
Canine	8	1	5	5	2	4
Feline	8	1	5	5	2	4
C.Elegans	8		4	5	2	4
Hamster	8		4	5	2	4
Plant	8		4	5	2	4
Guinea Pig	8	1	5	5	2	4
Fish	8		4	5	2	4
Eukaryotes	8		4	5	2	4
Rabbit	8	1	5	5	2	4
Xenopus	8	1	5	5	2	4
Donkey	8	1	5	5	2	4
Zebrafish	8		4	5	2	4
Prokaryotes	8		4	5	2	4
Porcine	8	1	5	5	2	4
Yeast	8		4	5	2	4
Squirrel	4		1	3	1	1
Vertebrates	4		1	3	1	1
Invertebrates	4		1	3	1	1
Duck	4		1	3	1	1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

57 matching His tag antibodies for Immunofluorescence from 29 suppliers

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His tag information

Aliases

1 Applications, 34 Species currently selected

57 matching His tag antibodies for Immunofluorescence after applying filters

Immunofluorescence - Use Filter to switch application

Western Blot

Related antibodies