Photomicrograph of mouse fibroblasts that have been formaldehyde-fixed, acetone-permeabilized and triple-stained with the F-actin-specific probe BODIPY FL phallacidin (B-607), with mouse monoclonal anti-beta-tubulin antibody in conjunction with Texas Red goat anti-mouse IgG (H+L) conjugate (T-862) and with DAPI (D-1306, D-3571). The image was obtained by taking multiple exposures through fluorescein, Texas Red and DAPI bandpass optical filter sets using a 100X Plan Apochromat objective. Omega Optical filter sets are available directly from Molecular Probes. Photo contributed by Molecular Probes, Inc. BODIPY and Texas Red are registered trademarks of Molecular Probes. Omega is a registered trademark of Omega Optical.

Transverse zebrafish retinal section that has been probed with FRet 43 (a monoclonal antibody that binds to double cones) and then developed for visualization with alkaline phosphatase-mediated techniques using our ELF-97 Immunohistochemistry Kit (E-6600). If this tissue had been stained with only the ELF-97 alkaline phosphatase substrate, the double-cone cells would appear uniformly bright green. However, this section has also been labeled with Hoechst 33342 (H-1399, H-3570), which stains nuclei blue, and with tetramethylrhodamine wheat germ agglutinin (TMR-WGA, W-849), which stains the photoreceptor outer segments and synaptic layers red. Thus, the double-cone outer segments and synaptic pedicles appear bright yellow because they are double-labeled with both the ELF-97 precipitate and TMR-WGA. Also, the ELF-97 precipitate-stained inner fibers of the double cones traverse the region occupied by the Hoechst 33342-stained rod nuclei, giving them a blue-green appearance. This image was obtained by double-exposing the film through DAPI and tetramethylrhodamine longpass optical filter sets. Photo contributed by Molecular Probes, Inc. ELF is a registered trademark of Molecular Probes.